Journal article
Mapping of an anthocyanin-regulating MYB transcription factor and its expression in red and green pear, Pyrus communis
Plant physiology and biochemistry, Vol.48(12), pp.1020-1026
2010
Handle:
https://hdl.handle.net/2376/110062
PMID: 20951056
Abstract
‘Max Red Bartlett’ is a red bud mutation of the yellow pear (
Pyrus communis L.) cultivar ‘Williams’ (known as ‘Bartlett’ in North America). Anthocyanins are the most important pigments for red colour in fruits. Synthesis of anthocyanins is mediated by a number of well-characterized enzymes that include chalcone synthase (CHS), flavanone-3-hydroxylase (F3H), dihydroflavonol-4-reductase (DFR), anthocyanidin synthase (ANS), and UDP-glucose:flavonoid-3-
O-glucosyltransferase (UFGT). Expression of the genes encoding these five enzymes was examined in pear fruit skin in order to elucidate the molecular mechanism for red coloration. In addition, the gene
PcMYB10, encoding an R2R3 MYB transcription factor involved in anthocyanin biosynthetic pathway regulation, was isolated from both ‘Williams’ and ‘Max Red Bartlett’. Analysis of the deduced amino acid sequence suggests that this gene is an ortholog of anthocyanin regulators known in other plant species. Its expression level was significantly higher in ‘Max Red Bartlett’ (red pear) compared with the original yellow variety ‘Williams’. Although the map position of
PcMYB10 corresponds to that of
MdMYBa and
MdMYB10, which control pigmentation of apple fruit skin,
PcMYB10 is not directly responsible for red versus yellow colour in the two pear varieties, as the mutation underlying this difference maps to a different region of the pear genome.
► The expression level of anthocyanin-related genes during fruit development of the European pear ‘Williams’ and its red-skinned sport ‘Max Red Bartlett’ was determined. ► The transcription factor PcMYB10 is most likely involved in the anthocyanin production in the early stages of ‘Max Red Bartlett’ fruit development. ► The red phenotype of ‘Max Red Bartlett’ however was not originated by a mutation of PcMYB10, as the gene does not co-map with the red colour trait.
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Details
- Title
- Mapping of an anthocyanin-regulating MYB transcription factor and its expression in red and green pear, Pyrus communis
- Creators
- Luca Pierantoni - Department of Fruit Tree and Woody Plant Sciences (DCA), University of Bologna, viale Fanin 46, 40127 Bologna, ItalyLuca Dondini - Department of Fruit Tree and Woody Plant Sciences (DCA), University of Bologna, viale Fanin 46, 40127 Bologna, ItalyPaolo De Franceschi - Department of Fruit Tree and Woody Plant Sciences (DCA), University of Bologna, viale Fanin 46, 40127 Bologna, ItalyStefano Musacchi - Department of Fruit Tree and Woody Plant Sciences (DCA), University of Bologna, viale Fanin 46, 40127 Bologna, ItalyBrenda S.J Winkel - Department of Biological Sciences, Virginia Tech, 118 N. Main St. Blacksburg, VA 24061, USASilviero Sansavini - Department of Fruit Tree and Woody Plant Sciences (DCA), University of Bologna, viale Fanin 46, 40127 Bologna, Italy
- Publication Details
- Plant physiology and biochemistry, Vol.48(12), pp.1020-1026
- Academic Unit
- Horticulture, Department of
- Publisher
- Elsevier Masson SAS
- Identifiers
- 99900547220901842
- Language
- English
- Resource Type
- Journal article