Journal article
Mathematical and numerical model to study two-dimensional free flow isoelectric focusing
Biomicrofluidics, Vol.8(3), pp.034111-034111
05/2014
Handle:
https://hdl.handle.net/2376/112640
PMCID: PMC4162414
PMID: 25379071
Abstract
Even though isoelectric focusing (IEF) is a very useful technique for sample concentration and
separation, it is challenging to extract separated samples for further processing. Moreover, the
continuous sample concentration and separation are not possible in the conventional IEF. To overcome
these challenges, free
flow
IEF (FFIEF) is introduced in which a
flow
field is applied in the
direction perpendicular to the applied
electric field.
In this study, a mathematical
model
is developed for FFIEF to understand
the roles of
flow
and
electric fields
for
efficient design of
microfluidic
chip
for continuous separation of
proteins
from an initial well mixed solution. A finite volume based
numerical scheme is implemented to simulate two dimensional FFIEF in a
microfluidic chip.
Simulation results
indicate that a pH gradient forms as samples
flow
downstream and this pH profile agrees well with
experimental results validating our
model.
In addition, our simulation results predict the experimental behavior of
p
I
markers in a FFIEF microchip. This numerical
model
is used to predict the separation
behavior of two
proteins
(serum albumin and cardiac troponin I) in a two-dimensional straight microchip. The effect of
electric field
is
investigated for continuous separation of
proteins.
Moreover, a new channel design is presented to increase the separation
resolution by introducing cross-stream
flow
velocity. Numerical results indicate that the separation
resolution can be improved by three folds in this new design compare to the conventional straight
channel design.
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Details
- Title
- Mathematical and numerical model to study two-dimensional free flow isoelectric focusing
- Creators
- Kisoo Yoo - School of Mechanical and Materials EngineeringJaesool Shim - School of Mechanical EngineeringJin Liu - School of Mechanical and Materials EngineeringPrashanta Dutta - School of Mechanical and Materials Engineering
- Publication Details
- Biomicrofluidics, Vol.8(3), pp.034111-034111
- Academic Unit
- Mechanical and Materials Engineering, School of
- Publisher
- American Institute of Physics
- Grant note
- CBET 1250107 / NSF
- Identifiers
- 99900547640401842
- Language
- English
- Resource Type
- Journal article