Journal article
Mcm10 and Cdc45 Cooperate in Origin Activation in Saccharomyces cerevisiae
Journal of molecular biology, Vol.340(2), pp.195-202
2004
Handle:
https://hdl.handle.net/2376/108714
PMID: 15201046
Abstract
Mcm10 has recently been found to play a crucial role in multiple steps of the DNA replication initiation process in eukaryotes. Here, we have examined the role of Mcm10 in assembling initiation factors at a well-characterized yeast replication origin, ARS1. We find that the pre-replication complex (pre-RC) components Cdc6 and Mcm7 associate with ARS1 in the
mcm10-1 mutant, suggesting that establishment of the pre-RC is not compromised in this mutant. Association of Cdc45 with ARS1 is reduced in the
mcm10-1 mutant, suggesting that Mcm10 is involved in recruiting Cdc45 to the pre-RC. We find that overexpression of either Mcm10-1 or Cdc45 suppresses the growth defect of
mcm10-1, and that a physical interaction between Cdc45 and Mcm10 is disrupted in the
mcm10-1 mutant. Our results show that interaction between the Mcm10 and Cdc45 proteins facilitates the recruitment of Cdc45 onto the ARS1 origin.
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Details
- Title
- Mcm10 and Cdc45 Cooperate in Origin Activation in Saccharomyces cerevisiae
- Creators
- Sara L SawyerIrene H ChengWeihang ChaiBik K Tye
- Publication Details
- Journal of molecular biology, Vol.340(2), pp.195-202
- Academic Unit
- UNKNOWN
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547755801842
- Language
- English
- Resource Type
- Journal article