Measured effect of collection and cooling conditions on the motility and the water transport parameters at subzero temperatures of equine spermatozoa

R V Devireddy; D J Swanlund; A S Alghamdi; L A Duoos; M H T Troedsson; J C Bischof; K P Roberts

doi:10.1530/rep.0.1240643

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Measured effect of collection and cooling conditions on the motility and the water transport parameters at subzero temperatures of equine spermatozoa

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Measured effect of collection and cooling conditions on the motility and the water transport parameters at subzero temperatures of equine spermatozoa

R V Devireddy, D J Swanlund, A S Alghamdi, L A Duoos, M H T Troedsson, J C Bischof and K P Roberts

Reproduction (Cambridge, England), Vol.124(5), pp.643-648

11/2002

DOI: https://doi.org/10.1530/rep.0.1240643

Handle:

https://hdl.handle.net/2376/110050

PMID: 12417002

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Abstract

Sperm Motility

Cryopreservation - veterinary

Water - metabolism

Male

Semen Preservation - veterinary

Cryopreservation - methods

Specimen Handling

Animals

Biological Transport

Horses

Spermatozoa - physiology

Cell Membrane - metabolism

Lubrication

Semen Preservation - methods

The effects of extracellular ice and cryoprotective agents on the measured volumetric shrinkage response and the membrane permeability parameters of equine spermatozoa have been reported previously. The volumetric shrinkage data were obtained using a differential scanning calorimeter technique that was independent of cell shape. The aim of this study was to examine the effects of collection and cooling conditions on the motility and the water transport parameters at subzero temperatures of equine spermatozoa. Stallion semen samples were collected using either a commercial lubricating agent, which caused osmotic stress to the spermatozoa, or water-insoluble Vaseline( trade mark ) as the artificial vagina lubricant. In some experiments, spermatozoa were cooled at 1 degrees C min(-1) from 20 degrees C to 4 degrees C to induce cold shock. An Equitainer was used to achieve control cooling rates (< or = 0.3 degrees C min(-1)) at temperatures > 0 degrees C. The water transport response of spermatozoa that were cold-shocked and osmotically shocked was significantly different from that of control spermatozoa (P < 0.01). Osmotic stress appeared to have an effect on the water transport response, although this effect was not significant. These results indicate that cold shock alters the behaviour of equine spermatozoa in cryopreservation protocols as a result of changes in the water transport properties of the plasma membrane. Although osmotic stress did not significantly affect water transport in equine spermatozoa, it did significantly decrease sperm motility in the extended semen samples (P < 0.01), which would, in turn, lower the quality of cold-stored or cryopreserved spermatozoa.

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Title: Measured effect of collection and cooling conditions on the motility and the water transport parameters at subzero temperatures of equine spermatozoa
Creators: R V Devireddy - Department of Mechanical Engineering, University of Minnesota, Minneapolis 55455, USA
D J Swanlund
A S Alghamdi
L A Duoos
M H T Troedsson
J C Bischof
K P Roberts
Publication Details: Reproduction (Cambridge, England), Vol.124(5), pp.643-648
Academic Unit: Elson S. Floyd College of Medicine
Publisher: England
Identifiers: 99900547765401842
Language: English
Resource Type: Journal article