Journal article
Measuring freezing tolerance: survival and regrowth assays
Methods in molecular biology (Clifton, N.J.), Vol.1166, pp.7-13
2014
Handle:
https://hdl.handle.net/2376/118091
PMID: 24852624
Abstract
Screening plants for freezing tolerance under tightly controlled conditions is an invaluable technique for studying freezing tolerance and selecting for improved winterhardiness. Artificial freezing tests of cereal plants historically have used isolated crown and stem tissue prepared by "removing all plant parts 3 cm above and 0.5 cm below the crown tissue" (Fowler et al., Crop Sci 21:896-901, 1981). Here, we describe a method of conducting freezing tolerance tests using intact plants grown in small horticultural containers, including suggested methods for collecting and analyzing the data.
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Details
- Title
- Measuring freezing tolerance: survival and regrowth assays
- Creators
- Daniel Z Skinner - USDA-ARS, Wheat Genetics Unit, Washington State University, 209 Johnson Hall, Pullman, WA, 99164, USA, dzs@wsu.eduKimberly Garland-Campbell
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.1166, pp.7-13
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- United States
- Identifiers
- 99900548454601842
- Language
- English
- Resource Type
- Journal article