Journal article
Mechanical property and in vitro biocompatibility of brushite cement modified by polyethylene glycol
Materials Science & Engineering C, Vol.32(8), pp.2145-2152
12/01/2012
Handle:
https://hdl.handle.net/2376/115485
PMID: 23139441
Abstract
Brushite (dicalcium phosphate dihydrate, DCPD) cement, owing to its high solubility in physiological condition and ability to guide new bone formation, is widely used to treat bone defects. In the present study, we have evaluated the effects of poly ethylene glycol (PEG) addition on the setting time, compressive strength and in vitro biocompatibility of brushite cement. The brushite cements were prepared by mixing β-tricalcium phosphate [β-TCP, Ca3(PO4)2] and monocalcium phosphate monohydrate [MCPM, Ca(H2PO4)2 ⋅ H2O]. PEG was introduced at 2.0 and 5.0wt% with the liquid. Introduction of PEG resulted in marginal increase in both initial and final setting time, however, significantly affected the compressive strength. Effects of PEG incorporation on in vitro biocompatibility of brushite cements were studied by using human fetal osteoblast cells (hFOB) cells. Field emission scanning electron microscope (FESEM) images and immunohistochemical analysis indicated that pure and PEG incorporated brushite cement facilitates cell adhesion, proliferation and differentiation. Fewer cells expressed vinculin protein with increased PEG content in the cement. Cell proliferation was found to decrease with increased PEG concentration while the cell differentiation increased with PEG content. Our results provide a better understanding of in vitro biocompatibility of PEG added brushite cements that can be used to customize the cement compositions based on application need.
► Setting time was not altered for brushite cement with PEG addition. ► hFOB cell proliferation was found to decrease with increased PEG concentration in brushite cement. ► Enhanced ALP activity was noticed with addition of PEG in brushite cements.
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Details
- Title
- Mechanical property and in vitro biocompatibility of brushite cement modified by polyethylene glycol
- Creators
- Mangal RoyKen DeVoeAmit BandyopadhyaySusmita Bose
- Publication Details
- Materials Science & Engineering C, Vol.32(8), pp.2145-2152
- Academic Unit
- Mechanical and Materials Engineering, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548268701842
- Language
- English
- Resource Type
- Journal article