Meiotic recombination in human oocytes

Edith Y Cheng; Patricia A Hunt; Theresa A Naluai-Cecchini; Corrine L Fligner; Victor Y Fujimoto; Tanya L Pasternack; Jackie M Schwartz; Jody E Steinauer; Tracey J Woodruff; Sheila M Cherry; Terah A Hansen; Rhea U Vallente; Karl W Broman; Terry J Hassold

doi:10.1371/journal.pgen.1000661

Back

Journal article

Open access

Meiotic recombination in human oocytes

Edith Y Cheng, Patricia A Hunt, Theresa A Naluai-Cecchini, Corrine L Fligner, Victor Y Fujimoto, Tanya L Pasternack, Jackie M Schwartz, Jody E Steinauer, Tracey J Woodruff, Sheila M Cherry, …

PLoS genetics, Vol.5(9), pp.e1000661-e1000661

09/2009

DOI: https://doi.org/10.1371/journal.pgen.1000661

Handle:

https://hdl.handle.net/2376/105733

PMCID: PMC2735652

PMID: 19763179

Files and links (1)

url

https://doi.org/10.1371/journal.pgen.1000661View

Published (Version of record) Open

Abstract

Sister Chromatid Exchange - genetics

MutL Protein Homolog 1

Confidence Intervals

Oocytes - metabolism

Chromosomes, Human - genetics

Humans

Nuclear Proteins - metabolism

Genome, Human - genetics

Protein Transport

Fetus - cytology

Young Adult

Meiosis - genetics

Oocytes - cytology

Trisomy - genetics

Time Factors

Recombination, Genetic

Adolescent

Adult

Female

Adaptor Proteins, Signal Transducing - metabolism

Studies of human trisomies indicate a remarkable relationship between abnormal meiotic recombination and subsequent nondisjunction at maternal meiosis I or II. Specifically, failure to recombine or recombination events located either too near to or too far from the centromere have been linked to the origin of human trisomies. It should be possible to identify these abnormal crossover configurations by using immunofluorescence methodology to directly examine the meiotic recombination process in the human female. Accordingly, we initiated studies of crossover-associated proteins (e.g., MLH1) in human fetal oocytes to analyze their number and distribution on nondisjunction-prone human chromosomes and, more generally, to characterize genome-wide levels of recombination in the human female. Our analyses indicate that the number of MLH1 foci is lower than predicted from genetic linkage analysis, but its localization pattern conforms to that expected for a crossover-associated protein. In studies of individual chromosomes, our observations provide evidence for the presence of "vulnerable" crossover configurations in the fetal oocyte, consistent with the idea that these are subsequently translated into nondisjunctional events in the adult oocyte.

Metrics

10 Record Views

Details

Title: Meiotic recombination in human oocytes
Creators: Edith Y Cheng - Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, United States of America
Patricia A Hunt
Theresa A Naluai-Cecchini
Corrine L Fligner
Victor Y Fujimoto
Tanya L Pasternack
Jackie M Schwartz
Jody E Steinauer
Tracey J Woodruff
Sheila M Cherry
Terah A Hansen
Rhea U Vallente
Karl W Broman
Terry J Hassold
Publication Details: PLoS genetics, Vol.5(9), pp.e1000661-e1000661
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: R01 ES013527 / NIEHS NIH HHS P20 ES018135 / NIEHS NIH HHS P01 HD013527 / NICHD NIH HHS HD13527 / NICHD NIH HHS HD21341 / NICHD NIH HHS R01 HD021341 / NICHD NIH HHS R37 HD021341 / NICHD NIH HHS
Identifiers: 99900547022301842
Language: English
Resource Type: Journal article