Mesoporous Pd@Pt nanoparticle-linked immunosorbent assay for detection of atrazine

Eunice Y. Kwon; Xiaofan Ruan; Limin Wang; Yuehe Lin; Dan Du; Bernard J. Van Wie

doi:10.1016/j.aca.2020.03.045

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Mesoporous Pd@Pt nanoparticle-linked immunosorbent assay for detection of atrazine

Journal article

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Mesoporous Pd@Pt nanoparticle-linked immunosorbent assay for detection of atrazine

Eunice Y. Kwon, Xiaofan Ruan, Limin Wang, Yuehe Lin, Dan Du and Bernard J. Van Wie

Analytica chimica acta, Vol.1116, pp.36-44

06/15/2020

DOI: https://doi.org/10.1016/j.aca.2020.03.045

PMID: 32389187

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Abstract

Chemistry

Chemistry, Analytical

Science & Technology

Physical Sciences

Atrazine is a widely used herbicide in the United States; however, the Environmental Protection Agency (EPA) has issued warnings about atrazine because of its reported potential harmful effects on animals and humans. Therefore, developing efficient ways to detect this herbicide's residue are critically important. The competitive ELISA is a useful method for detecting chemicals for which antibodies exist due to its high sensitivity, specificity, and efficiency. However, the assay typically requires a separate application of a secondary antibody linked to an enzyme that catalyzes conversion of a non-colored organic to a detectable colored product. In this study, we used the recently developed peroxidase-like mesoporous core-shell palladium@-platinum (Pd@Pt) nanoparticle which can easily be bound directly to primary antibody, thereby eliminating the need for a secondary antibody conjugate. We report a first instance in which this technique is applied for use in a competitive assay for small molecules, in this case the herbicide atrazine. Due to their high-surface area and mesoporous structure, Pd@Pt nanoparticles enable fast mass transfer for reaction with excellent catalytic activity. This leads to high sensitivity in our immunoassay with a limit of detection of 0.5 ng mL(-1) defined by selecting an IC50 concentration, i.e., the analyte concentration at which 10% of the available Pd@Pt nanoparticle-labeled antibody is inhibited from binding to a plate coated with a bovine serum albumin-atrazine conjugate. We applied our method to well-water and pond water samples spiked with atrazine. Our tests at 5,10, and 20 ng mL(-1) yielded recoveries of 99 - 115%, offering strong supporting evidence that atrazine and other low molecular weight herbicides and pesticides can be detected using this immunoassay approach. Detection with this method is expected to lead to its use in a wide spectrum of applications in agriculture, medical, and biotechnology arenas. (C) 2020 Elsevier B.V. All rights reserved.

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Title: Mesoporous Pd@Pt nanoparticle-linked immunosorbent assay for detection of atrazine
Creators: Eunice Y. Kwon - Washington State University
Xiaofan Ruan - Washington State University
Limin Wang - Washington State University
Yuehe Lin - Washington State University
Dan Du - Washington State University
Bernard J. Van Wie - Washington State University
Publication Details: Analytica chimica acta, Vol.1116, pp.36-44
Academic Unit: School of Mechanical and Materials Engineering
Publisher: Elsevier
Number of pages: 9
Grant note: 10646-0239 / Washington State University NASA Space Grant Consortium Fellowship, Encouraging Excellence in Space-Related Science 2018-67021-27970 / United States Department of Agriculture (USDA) National Institute of Food and Agriculture, AFRI project
Identifiers: 99901227849301842
Language: English
Resource Type: Journal article