Journal article
Metabolic Engineering of Oleaginous Yeast Yarrowia lipolytica for Overproduction of Fatty Acids
Frontiers in microbiology, Vol.11(11), pp.1717-1717
07/24/2020
Handle:
https://hdl.handle.net/2376/110890
Abstract
The oleaginous yeast
Yarrowia lipolytica
has attracted much attention due to its ability to utilize a wide range of substrates to accumulate high lipid content and its flexibility for genetic manipulation. In this study, intracellular lipid metabolism in
Y. lipolytica
was tailored to produce fatty acid, a renewable oleochemical and precursor for production of advanced biofuels. Two main strategies, including blocking activation and peroxisomal uptake of fatty acids and elimination of biosynthesis of lipids, were employed to reduce fatty acid consumption by the native pathways in
Y. lipolytica
. Both genetic modifications improved fatty acid production. However, disruption of the genes responsible for assembly of nonpolar lipid molecules including triacylglycerols (TAGs) and steryl esters resulted in the deleterious effects on the cell growth. The gene
tesA
encoding thioesterase from
Escherichia coli
was expressed in the strain with disrupted
faa
genes encoding fatty acyl-CoA synthetases and
pxa1
encoding peroxisomal acyl-CoA transporter, and the titer of fatty acids resulted in 2.3 g/L in shake flask culture, representing 11-fold improvement compared with the parent strain. Expressing the native genes encoding acetyl-CoA carboxylase (ACC) and hexokinase also increased fatty acid production, although the improvement was not as significant as that with
tesA
expression. Saturated fatty acids including palmitic acid (C16:0) and stearic acid (C18:0) increased remarkably in the fatty acid composition of the recombinant bearing
tesA
compared with the parent strain. The recombinant expressing
tesA
gene resulted in high lipid content, indicating the great fatty acid producing potential of
Y. lipolytica
. The results highlight the achievement of fatty acid overproduction without adverse effect on growth of the strain. Results of this study provided insight into the relationship between fatty acid and lipid metabolism in
Y. lipolytica
, confirming the avenue to reprogram lipid metabolism of this host for overproduction of renewable fatty acids.
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Details
- Title
- Metabolic Engineering of Oleaginous Yeast Yarrowia lipolytica for Overproduction of Fatty Acids
- Creators
- Rishikesh GhogareShulin ChenXiaochao Xiong
- Publication Details
- Frontiers in microbiology, Vol.11(11), pp.1717-1717
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- Frontiers Media S.A
- Grant note
- WNP00009 / USDA National Institute of Food and Agriculture
- Identifiers
- 99900583053901842
- Copyright
- Copyright © 2020 Ghogare, Chen and Xiong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Language
- English
- Resource Type
- Journal article