Journal article
Metabolic Profiling of Escherichia coli by Ion Mobility-Mass Spectrometry with MALDI Ion Source
Journal of mass spectrometry., Vol.45(12), pp.1383-1393
12/2010
Handle:
https://hdl.handle.net/2376/102591
PMCID: PMC3012737
PMID: 20967735
Abstract
Comprehensive metabolome analysis using mass spectrometry (MS) often results in a complex mass spectrum and difficult data analysis resulting from the signals of numerous small molecules in the metabolome. In addition, mass spectrometry alone has difficulty measuring isobars and chiral, conformational, and structural isomers. When a matrix assisted laser desorption ionization source (MALDI) is added, the difficulty and complexity are further increased. Signal interference between analyte signals and matrix ion signals produced by MALDI in the low mass region (<1500 Da) cause detection and or identification of metabolites difficult by mass spectrometry alone.
However, ion mobility spectrometry (IMS) coupled with MS (IM-MS) provides a rapid analytical tool for measuring subtle structural differences in chemicals. IMS separates gas phase ions based on their size-to-charge ratio. This study, for the first time, reports the application of MALDI to the measurement of small molecules in a biological matrix by Ion Mobility-Time of Flight Mass Spectrometry (IM-TOFMS) and demonstrates the advantage of ion-signal dispersion in the second dimension. Qualitative comparisons between metabolic profiling of the
Escherichia coli
metabolome by MALDI-TOFMS, MALDI-IM-TOFMS, and ESI-IM-TOFMS (electrospray ionization) are reported. Results demonstrate that mobility separation prior to mass analysis increases peak-capacity through added dimensionality in measurement. Mobility separation also allows detection of metabolites in the matrix-ion dominated low-mass range (
m/z
< 1500 Da) by separating matrix-signals from non-matrix signals in mobility space.
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Details
- Title
- Metabolic Profiling of Escherichia coli by Ion Mobility-Mass Spectrometry with MALDI Ion Source
- Creators
- Prabha Dwivedi - Washington State University, Pullman, WA, 99163 USAGeoffery Puzon - CSIRO, Land and Water, Private Bag No 5, Wembley, WA, AUS 6913Maggie Tam - Washington State University, Pullman, WA, 99163 USADenis Langlais - Ionwerks Inc., Houston, TX, 77005 USAShelley Jackson - NIDA IRP, NIH, Baltimore, MD, 21224William F Siems - Washington State University, Pullman, WA, 99163 USAAlbert J Schultz - Ionwerks Inc., Houston, TX, 77005 USALuying Xun - Washington State University, Pullman, WA, 99163 USAAmina Woods - NIDA IRP, NIH, Baltimore, MD, 21224Herbert H Hill - Washington State University, Pullman, WA, 99163 USA
- Publication Details
- Journal of mass spectrometry., Vol.45(12), pp.1383-1393
- Academic Unit
- Chemistry, Department of; Molecular Biosciences, School of
- Identifiers
- 99900546616501842
- Language
- English
- Resource Type
- Journal article