Journal article
Metabolic engineering of enhanced glycerol-3-phosphate synthesis to increase lipid production in Synechocystis sp. PCC 6803
Applied microbiology and biotechnology, Vol.100(13), pp.6091-6101
07/2016
Handle:
https://hdl.handle.net/2376/117995
PMID: 27154348
Abstract
With the growing attention to global warming and energy sustainability, biosynthesis of lipids by photosynthetic microorganisms has attracted more interest for the production of renewable transportation fuels. Recently, the cyanobacterium Synechocystis sp. PCC 6803 has been widely used for biofuel production through metabolic engineering because of its efficient photosynthesis and well-developed genetic tools. In lipid biosynthesis, glycerol-3-phosphate (G3P) is a key node for both CO2 fixation and lipid metabolism in cyanobacteria. However, few studies have explored the use of G3P synthesis to improve photosynthetic lipid production. In this study, metabolic engineering combined with flux balance analysis (FBA) was conducted to reveal the effect of G3P synthesis on lipid production. Heterologous genes that encoded glycerol-3-phosphate dehydrogenase (GPD) and diacylglycerol acyltransferase (DGAT) were engineered into Synechocystis sp. PCC 6803 to enhance G3P supply and lipid production. The resultant recombinant Synechocystis produced higher levels of lipids without a significant reduction in cell growth. Compared with the wild-type strain, lipid content and productivity of the engineered cyanobacteria increased by up to 36 and 31 %, respectively, under autotrophic conditions. Lipid production under mixotrophic conditions of the engineered cyanobacteria was also investigated. This work demonstrated that enhanced G3P synthesis was an important factor in photosynthetic lipid production and that introducing heterologous GPD and DGAT genes was an effective strategy to increase lipid production in Synechocystis sp. PCC 6803.
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Details
- Title
- Metabolic engineering of enhanced glycerol-3-phosphate synthesis to increase lipid production in Synechocystis sp. PCC 6803
- Creators
- Xi Wang - Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164, USAXiaochao Xiong - Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164, USANa Sa - Institute of Biological Chemistry, Washington State University, Pullman, WA, 99164, USASanja Roje - Institute of Biological Chemistry, Washington State University, Pullman, WA, 99164, USAShulin Chen - Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164, USA. chens@wsu.edu
- Publication Details
- Applied microbiology and biotechnology, Vol.100(13), pp.6091-6101
- Identifiers
- 99900582336601842
- Language
- English
- Resource Type
- Journal article