Journal article
Metabolic profiling and phylogenetic analysis of medicinal Zingiber species: Tools for authentication of ginger ( Zingiber officinale Rosc.)
Phytochemistry (Oxford), Vol.67(15), pp.1673-1685
2006
Handle:
https://hdl.handle.net/2376/114554
PMID: 16169024
Abstract
Molecular and chemical data can be used to distinguish ginger from other medicinal plants in
Zingiber. In addition, phylogeny based on the DNA sequences (A) matched closely the phylogeny based on chemical profiles (B) for these
Zingiber species.
Phylogenetic analysis and metabolic profiling were used to investigate the diversity of plant material within the ginger species and between ginger and closely related species in the genus
Zingiber (Zingiberaceae). In addition, anti-inflammatory data were obtained for the investigated species. Phylogenetic analysis demonstrated that all
Zingiber officinale samples from different geographical origins were genetically indistinguishable. In contrast, other
Zingiber species were significantly divergent, allowing all species to be clearly distinguished using this analysis. In the metabolic profiling analysis, the
Z. officinale samples derived from different origins showed no qualitative differences in major volatile compounds, although they did show some significant quantitative differences in non-volatile composition, particularly regarding the content of [6]-, [8]-, and [10]-gingerols, the most active anti-inflammatory components in this species. The differences in gingerol content were verified by HPLC. The metabolic profiles of other
Zingiber species were very different, both qualitatively and quantitatively, when compared to
Z. officinale and to each other. Comparative DNA sequence/chemotaxonomic phylogenetic trees showed that the chemical characters of the investigated species were able to generate essentially the same phylogenetic relationships as the DNA sequences. This supports the contention that chemical characters can be used effectively to identify relationships between plant species. Anti-inflammatory in vitro assays to evaluate the ability of all extracts from the
Zingiber species examined to inhibit LPS-induced PGE
2 and TNF-α production suggested that bioactivity may not be easily predicted by either phylogenetic analysis or gross metabolic profiling. Therefore, identification and quantification of the actual bioactive compounds are required to guarantee the bioactivity of a particular
Zingiber sample even after performing authentication by molecular and/or chemical markers.
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Details
- Title
- Metabolic profiling and phylogenetic analysis of medicinal Zingiber species: Tools for authentication of ginger ( Zingiber officinale Rosc.)
- Creators
- Hongliang Jiang - Arizona Center for Phytomedicine Research, College of Pharmacy, University of Arizona, USAZhengzhi Xie - Arizona Center for Phytomedicine Research, College of Pharmacy, University of Arizona, USAHyun Jo Koo - Arizona Center for Phytomedicine Research, College of Pharmacy, University of Arizona, USASteven P McLaughlin - Arizona Center for Phytomedicine Research, College of Pharmacy, University of Arizona, USABarbara N Timmermann - Arizona Center for Phytomedicine Research, College of Pharmacy, University of Arizona, USADavid R Gang - Arizona Center for Phytomedicine Research, College of Pharmacy, University of Arizona, USA
- Publication Details
- Phytochemistry (Oxford), Vol.67(15), pp.1673-1685
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900548215501842
- Language
- English
- Resource Type
- Journal article