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CC BY V4.0, Open Access
Journal article
Methane oxidation in anoxic lake water stimulated by nitrate and sulfate addition
Environmental microbiology, Vol.22(2), pp.766-782
02/01/2020
Handle:
https://hdl.handle.net/2376/123504
PMCID: PMC7027835
PMID: 31814267
Abstract
Methanotrophic bacteria play a key role in limiting methane emissions from lakes. It is generally assumed that methanotrophic bacteria are mostly active at the oxic-anoxic transition zone in stratified lakes, where they use oxygen to oxidize methane. Here, we describe a methanotroph of the genera Methylobacter that is performing high-rate (up to 72 mu M day(-1)) methane oxidation in the anoxic hypolimnion of the temperate Lacamas Lake (Washington, USA), stimulated by both nitrate and sulfate addition. Oxic and anoxic incubations both showed active methane oxidation by a Methylobacter species, with anoxic rates being threefold higher. In anoxic incubations, Methylobacter cell numbers increased almost two orders of magnitude within 3 days, suggesting that this specific Methylobacter species is a facultative anaerobe with a rapid response capability. Genomic analysis revealed adaptations to oxygen-limitation as well as pathways for mixed-acid fermentation and H-2 production. The denitrification pathway was incomplete, lacking the genes narG/napA and nosZ, allowing only for methane oxidation coupled to nitrite-reduction. Our data suggest that Methylobacter can be an important driver of the conversion of methane in oxygen-limited lake systems and potentially use alternative electron acceptors or fermentation to remain active under oxygen-depleted conditions.
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Details
- Title
- Methane oxidation in anoxic lake water stimulated by nitrate and sulfate addition
- Creators
- Sigrid van Grinsven - Utrecht UniversityJaap S. Sinninghe Damste - Utrecht UniversityAlejandro Abdala Asbun - Utrecht UniversityJulia C Engelmann - Utrecht UniversityJohn Harrison - Washington State UniversityLaura Villanueva - Utrecht University
- Publication Details
- Environmental microbiology, Vol.22(2), pp.766-782
- Academic Unit
- Harrison Research Group: Global Change and Watershed Biochemistry ; Environment, School of the (CAS)
- Publisher
- WILEY
- Number of pages
- 17
- Grant note
- Netherlands Organisation for Scientific Research (NWO) 024.002.002 / Soehngen Institute of Anaerobic Microbiology (SIAM) Gravitation grant
- Identifiers
- 99900667900101842
- Language
- English
- Resource Type
- Journal article