Journal article
Methyl parathion hydrolase based nanocomposite biosensors for highly sensitive and selective determination of methyl parathion
Biosensors & bioelectronics, Vol.26(11), pp.4320-4325
2011
Handle:
https://hdl.handle.net/2376/110435
PMID: 21616655
Abstract
This article reports the fabrication of a nanocomposite biosensor for the sensitive and specific detection of methyl parathion. The nanocomposite sensing film was prepared via the formation of gold nanoparticles on silica particles, mixing with multiwall carbon nanotubes and subsequent covalent immobilization of methyl parathion hydrolase. The composite of the individual materials was finely tuned to offer the sensing film with high specific surface area and high conductivity. A significant synergistic effect of nanocomposites on the biosensor performance was observed in biosensing methyl parathion. The square wave voltammetric responses displayed well defined peaks, linearly proportional to the concentrations of methyl parathion in the range from 0.001
μg
mL
−1 to 5.0
μg
mL
−1 with a detection limit of 0.3
ng
mL
−1. The application of this biosensor in the analysis of spiked garlic samples was also evaluated. The proposed protocol can be used as a platform for the simple and fast construction of biosensors with good performance for the determination of enzyme-specific electroactive species.
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Details
- Title
- Methyl parathion hydrolase based nanocomposite biosensors for highly sensitive and selective determination of methyl parathion
- Creators
- Shizhen Chen - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Chemistry College, Central China Normal University, Luoyu Str. 152, Wuhan 430079, Hubei Province, PR ChinaJing Huang - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Chemistry College, Central China Normal University, Luoyu Str. 152, Wuhan 430079, Hubei Province, PR ChinaDan Du - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Chemistry College, Central China Normal University, Luoyu Str. 152, Wuhan 430079, Hubei Province, PR ChinaJinlin Li - Key Laboratory of Catalysis and Materials Science of the State Ethnic Affairs Commision & Ministry of Education, South-Central University for Nationalities, Wuhan 430074, PR ChinaHaiyang Tu - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Chemistry College, Central China Normal University, Luoyu Str. 152, Wuhan 430079, Hubei Province, PR ChinaDeli Liu - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Chemistry College, Central China Normal University, Luoyu Str. 152, Wuhan 430079, Hubei Province, PR ChinaAidong Zhang - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Chemistry College, Central China Normal University, Luoyu Str. 152, Wuhan 430079, Hubei Province, PR China
- Publication Details
- Biosensors & bioelectronics, Vol.26(11), pp.4320-4325
- Academic Unit
- School of Mechanical and Materials Engineering; School of Chemical Engineering and Bioengineering
- Publisher
- Elsevier B.V
- Identifiers
- 99900547140301842
- Language
- English
- Resource Type
- Journal article