Journal article
Micro-/nanoscale electroporation
Lab on a chip, Vol.16(21), pp.4047-462
10/18/2016
Handle:
https://hdl.handle.net/2376/106944
PMID: 27713986
Abstract
Electroporation has been one of the most popular non-viral technologies for cell transfection. However, conventional bulk electroporation (BEP) shows significant limitations in efficiency, cell viability and transfection uniformity. Recent advances in microscale-electroporation (MEP) resulted in improved cell viability. Further miniaturization of the electroporation system (i.e., nanoscale) has brought up many unique advantages, including negligible cell damage and dosage control capabilities with single-cell resolution, which has enabled more translational applications. In this review, we give an insight into the fundamental and technical aspects of micro- and nanoscale/nanochannel electroporation (NEP) and go over several examples of MEP/NEP-based cutting-edge research, including gene editing, adoptive immunotherapy, and cellular reprogramming. The challenges and opportunities of advanced electroporation technologies are also discussed.
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Details
- Title
- Micro-/nanoscale electroporation
- Creators
- Lingqian Chang - Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA. lee.31@osu.edu gallegoperez.1@osu.eduLei Li - School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USAJunfeng Shi - Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USAYan Sheng - William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43209, USAWu Lu - Department of Electrical and Computer Engineering, The Ohio State University, Columbus, OH 43209, USADaniel Gallego-Perez - Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA. lee.31@osu.edu gallegoperez.1@osu.edu and Department of Surgery, The Ohio State University, Columbus, OH 43210, USALy James Lee - Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA. lee.31@osu.edu gallegoperez.1@osu.edu and Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA and William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43209, USA
- Publication Details
- Lab on a chip, Vol.16(21), pp.4047-462
- Academic Unit
- UNKNOWN
- Publisher
- England
- Identifiers
- 99900546963201842
- Language
- English
- Resource Type
- Journal article