Journal article
MicroRNA 132 alters sleep and varies with time in brain
Journal of applied physiology (1985), Vol.111(3), pp.665-672
09/2011
Handle:
https://hdl.handle.net/2376/104405
PMCID: PMC3174793
PMID: 21719725
Abstract
MicroRNA (miRNA) levels in brain are altered by sleep deprivation; however, the direct effects of any miRNA on sleep have not heretofore been described. We report herein that intracerebroventricular application of a miRNA-132 mimetic (preMIR-132) decreased duration of non-rapid-eye-movement sleep (NREMS) while simultaneously increasing duration of rapid eye movement sleep (REMS) during the light phase. Further, preMIR-132 decreased electroencephalographic (EEG) slow-wave activity (SWA) during NREMS, an index of sleep intensity. In separate experiments unilateral supracortical application of preMIR-132 ipsilaterally decreased EEG SWA during NREMS but did not alter global sleep duration. In addition, after ventricular or supracortical injections of preMIR-132, the mimetic-induced effects were state specific, occurring only during NREMS. After local supracortical injections of the mimetic, cortical miRNA-132 levels were higher at the time sleep-related EEG effects were manifest. We also report that spontaneous cortical levels of miRNA-132 were lower at the end of the sleep-dominant light period compared with at the end of the dark period in rats. Results suggest that miRNAs play a regulatory role in sleep and provide a new tool for investigating sleep regulation.
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Details
- Title
- MicroRNA 132 alters sleep and varies with time in brain
- Creators
- Christopher J Davis - Sleep and Performance Research Center, Program in Neuroscience, and WWAMI Medical Education, Washington State University-Spokane, Spokane, WashingtonJames M Clinton - Sleep and Performance Research Center, Program in Neuroscience, and WWAMI Medical Education, Washington State University-Spokane, Spokane, WashingtonPing Taishi - Sleep and Performance Research Center, Program in Neuroscience, and WWAMI Medical Education, Washington State University-Spokane, Spokane, WashingtonStewart G Bohnet - Sleep and Performance Research Center, Program in Neuroscience, and WWAMI Medical Education, Washington State University-Spokane, Spokane, WashingtonKimberly A Honn - Sleep and Performance Research Center, Program in Neuroscience, and WWAMI Medical Education, Washington State University-Spokane, Spokane, WashingtonJames M Krueger - Sleep and Performance Research Center, Program in Neuroscience, and WWAMI Medical Education, Washington State University-Spokane, Spokane, Washington
- Publication Details
- Journal of applied physiology (1985), Vol.111(3), pp.665-672
- Academic Unit
- Biomedical Sciences, Department of; Integrative Physiology and Neuroscience, Department of
- Identifiers
- 99900546742801842
- Language
- English
- Resource Type
- Journal article