Journal article
Microchannel protein separation by electric field gradient focusing
Lab on a chip, Vol.5(6), pp.587-597
06/2005
Handle:
https://hdl.handle.net/2376/115025
PMID: 15915250
Abstract
A microchannel device is presented which separates and focuses charged proteins based on electric field gradient focusing. Separation is achieved by setting a constant electroosmotic flow velocity against step changes in electrophoretic velocity. Where these two velocities are balanced for a given analyte, the analyte focuses at that point because it is driven to it from all points within the channel. We demonstrate the separation and focusing of a binary mixture of bovine serum albumin and phycoerythrin. The device is constructed of intersecting microchannels in poly(dimethylsiloxane)(PDMS) inlaid with hollow dialysis fibers. The device uses no exotic chemicals such as antibodies or synthetic ampholytes, but operates instead by purely physical means involving the independent manipulation of electrophoretic and electroosmotic velocities. One important difference between this apparatus and most other devices designed for field-gradient focusing is the injection of current at discrete intersections in the channel rather than continuously along the length of a membrane-bound separation channel.
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Details
- Title
- Microchannel protein separation by electric field gradient focusing
- Creators
- Dimiter N Petsev - Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque 87131, USAGabriel P LopezCornelius F IvoryScott S Sibbett
- Publication Details
- Lab on a chip, Vol.5(6), pp.587-597
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- England
- Identifiers
- 99900548395401842
- Language
- English
- Resource Type
- Journal article