Journal article
Microfluidic one-step synthesis of alginate microspheres immobilized with antibodies
Journal of the Royal Society interface, Vol.10(88), pp.20130566-20130566
11/06/2013
Handle:
https://hdl.handle.net/2376/105955
PMCID: PMC3785821
PMID: 23966617
Abstract
Micrometre- and submicrometre-size functionalized beads are frequently used to capture targets of interest from a biological sample for biological characterizations and disease diagnosis. The main challenge of the microbead-based assay is in the immobilization of probe molecules onto the microbead surfaces. In this paper, we report a versatile droplet microfluidics method to fabricate alginate microspheres while simultaneously immobilizing anti-
Mycobacterium tuberculosis
complex IgY and anti-
Escherichia coli
IgG antibodies primarily on the porous alginate carriers for specific binding and binding affinity tests. The binding affinity of antibodies is directly measured by fluorescence intensity of stained target bacteria on the microspheres. We demonstrate that the functionalized alginate microspheres yield specificity comparable with an enzyme-linked immunosorbent assay. The high surface area-to-volume ratio of the functionalized porous alginate microspheres improves the detection limit. By using the droplet microfluidics, we can easily modify the size and shape of alginate microspheres, and increase the concentration of functionalized alginate microspheres to further enhance binding kinetics and enable multiplexing.
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Details
- Title
- Microfluidic one-step synthesis of alginate microspheres immobilized with antibodies
- Creators
- Wanyu Chen - University of WashingtonJong-Hoon Kim - University of WashingtonDi Zhang - University of WashingtonKyong-Hoon Lee - University of WashingtonG. A Cangelosi - University of WashingtonS. D Soelberg - University of WashingtonC. E Furlong - University of WashingtonJae-Hyun Chung - University of WashingtonAmy Q Shen - University of Washington
- Publication Details
- Journal of the Royal Society interface, Vol.10(88), pp.20130566-20130566
- Publisher
- The Royal Society
- Identifiers
- 99900546967001842
- Language
- English
- Resource Type
- Journal article