Mitochondria thioredoxin's backup role in oxidative stress resistance in Trichoderma reesei

Guokun Wang; Haijun Wang; Xiaochao Xiong; Shulin Chen; Dongyuan Zhang

doi:10.1016/j.micres.2015.01.005

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Mitochondria thioredoxin's backup role in oxidative stress resistance in Trichoderma reesei

Guokun Wang, Haijun Wang, Xiaochao Xiong, Shulin Chen and Dongyuan Zhang

Microbiological research, Vol.171, pp.32-38

02/2015

DOI: https://doi.org/10.1016/j.micres.2015.01.005

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https://hdl.handle.net/2376/108301

PMID: 25644950

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Abstract

Mitochondria

Reactive oxygen species

Thioredoxin

Trichoderma reesei

Glutathione

Microorganisms often suffer from oxidative stress created from nutrient starvation and environmental changes. Thioredoxin (Trx) and glutathione (GSH) pathways are believed critical in related protective functions. The roles of Trx in improving abiotic stress resistance in Trichoderma reesei are still unclear. In this study, we identified a Trx-encoding gene, Trtrx1. The protein expressed located specifically in the mitochondria as verified by the fluorescence signals of TrTRX1-EGFP. TrTRX1 can catalyze the reduction of insulin disulfides by dithiothreitol (DTT). Loss of Trtrx1 however, did not lead to either significant morphology abnormality under normal and oxidative stress condition, or detectable difference in reactive oxygen species (ROS) resistance. The unchanged GSH amount in Trtrx1 deletion strain under normal condition and slight increase under oxidative stress condition, as well as the interplay between Trx and GSH systems suggested that GSH system was dominant and sufficient to maintain the mitochondrial redox state in T. reesei, where TrTRX1 played a role as the backup oxidative stress resistance.

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Title: Mitochondria thioredoxin's backup role in oxidative stress resistance in Trichoderma reesei
Creators: Guokun Wang - Tianjin Key Laboratory of Industrial Biosystem and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, PR China
Haijun Wang - Tianjin Key Laboratory of Industrial Biosystem and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, PR China
Xiaochao Xiong - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USA
Shulin Chen - Tianjin Key Laboratory of Industrial Biosystem and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, PR China
Dongyuan Zhang - Tianjin Key Laboratory of Industrial Biosystem and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, PR China
Publication Details: Microbiological research, Vol.171, pp.32-38
Academic Unit: Biological Systems Engineering, Department of
Publisher: Elsevier GmbH
Identifiers: 99900583053201842
Language: English
Resource Type: Journal article