Journal article
Modeling biofilms with dual extracellular electron transfer mechanisms
Physical chemistry chemical physics : PCCP, Vol.15(44), pp.19262-19283
11/28/2013
Handle:
https://hdl.handle.net/2376/106905
PMCID: PMC3868370
PMID: 24113651
Abstract
Electrochemically active biofilms have a unique form of respiration in which they utilize solid external materials as terminal electron acceptors for their metabolism. Currently, two primary mechanisms have been identified for long-range extracellular electron transfer (EET): a diffusion- and a conduction-based mechanism. Evidence in the literature suggests that some biofilms, particularly
Shewanella oneidensis
, produce the requisite components for both mechanisms. In this study, a generic model is presented that incorporates the diffusion- and the conduction-based mechanisms and allows electrochemically active biofilms to utilize both simultaneously. The model was applied to
S. oneidensis
and
Geobacter sulfurreducens
biofilms using experimentally generated data found in the literature. Our simulation results show that 1) biofilms having both mechanisms available, especially if they can interact, may have a metabolic advantage over biofilms that can use only a single mechanism; 2) the thickness of
G. sulfurreducens
biofilms is likely not limited by conductivity; 3) accurate intrabiofilm diffusion coefficient values are critical for current generation predictions; and 4) the local biofilm potential and redox potential are two distinct parameters and cannot be assumed to have identical values. Finally, we determined that simulated cyclic and squarewave voltammetry based on our model are currently not capable of determining the specific percentages of extracellular electron transfer mechanisms in a biofilm. The developed model will be a critical tool for designing experiments to explain EET mechanisms.
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Details
- Title
- Modeling biofilms with dual extracellular electron transfer mechanisms
- Creators
- Ryan Renslow - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, United States of AmericaJerome Babauta - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, United States of AmericaAndrew Kuprat - Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, United States of AmericaJim Schenk - The Department of Chemistry, Washington State University, Pullman, WA, United States of AmericaCornelius Ivory - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, United States of AmericaJim Fredrickson - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States of AmericaHaluk Beyenal - The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, United States of America
- Publication Details
- Physical chemistry chemical physics : PCCP, Vol.15(44), pp.19262-19283
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Identifiers
- 99900546936701842
- Language
- English
- Resource Type
- Journal article