Journal article
Molecular Characterization of Lal2, an SRK-Like Gene Linked to the S-Locus in the Wild Mustard Leavenworthia alabamica
Genetics (Austin), Vol.178(4), pp.2055-2067
04/2008
Handle:
https://hdl.handle.net/2376/114155
PMCID: PMC2323796
PMID: 18430933
Abstract
Single-locus sporophytic self-incompatibility inhibits inbreeding in many members of the mustard family (Brassicaceae). To investigate the genetics of self-incompatibility in the wild mustard
Leavenworthia alabamica
, diallel crosses were conducted between full siblings. Patterns of incompatibility were consistent with the action of single-locus sporophytic self-incompatibility. DNA sequences related to
S-locus receptor kinase
(
SRK
), the gene involved in self-pollen recognition in mustards, were cloned and sequenced. A single sequence with high identity to
SRK
and several other groups of sequences (
Lal1
,
Lal2
,
Lal3
,
Lal8
, and
Lal14
) were isolated from
L. alabamica
. We propose that either
Lal2
sequences are divergent alleles of
SRK
or
Lal2
is in tight linkage with
SRK
because (1)
Lal2
alleles cosegregate with S-alleles inferred from dialleles in all 97 cases tested in five families; (2)
Lal2
sequences are highly diverse at both synonymous and nonsynonymous sites and exhibit patterns of selective constraint similar to those observed at
SRK
in Brassica and Arabidopsis; and (3) transcripts of one
Lal2
allele were detected in leaves and the styles of open flowers, but were most abundant in the stigmas of maturing buds. We discuss the utility of the S-linked polymorphism at
Lal2
for studying the evolutionary forces acting on self-incompatibility in Leavenworthia.
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Details
- Title
- Molecular Characterization of Lal2, an SRK-Like Gene Linked to the S-Locus in the Wild Mustard Leavenworthia alabamica
- Creators
- Jeremiah W Busch - Department of Biology, McGill University, Montreal, Quebec H3A 1B1, CanadaJulia Sharma - Department of Biology, McGill University, Montreal, Quebec H3A 1B1, CanadaDaniel J Schoen - Department of Biology, McGill University, Montreal, Quebec H3A 1B1, Canada
- Publication Details
- Genetics (Austin), Vol.178(4), pp.2055-2067
- Academic Unit
- Biological Sciences, School of
- Publisher
- Genetics Society of America
- Identifiers
- 99900547950101842
- Language
- English
- Resource Type
- Journal article