Journal article
Molecular Mechanisms of Pharmaceutical Drug Binding into Calsequestrin
International journal of molecular sciences, Vol.13(11), pp.14326-14343
2012
Handle:
https://hdl.handle.net/2376/105857
PMCID: PMC3509583
PMID: 23203067
Abstract
Calsequestrin (CASQ) is a major Ca
2+
-storage/buffer protein present in the sarcoplasmic reticulum of both skeletal (CASQ1) and cardiac (CASQ2) muscles. CASQ has significant affinity for a number of pharmaceutical drugs with known muscular toxicities. Our approach, with
in silico
molecular docking, single crystal X-ray diffraction, and isothermal titration calorimetry (ITC), identified three distinct binding pockets on the surface of CASQ2, which overlap with 2-methyl-2,4-pentanediol (MPD) binding sites observed in the crystal structure. Those three receptor sites based on canine CASQ1 crystal structure gave a high correlation (
R
2
= 0.80) to our ITC data. Daunomycin, doxorubicin, thioridazine, and trifluoperazine showed strong affinity to the S1 site, which is a central cavity formed between three domains of CASQ2. Some of the moderate-affinity drugs and some high-affinity drugs like amlodipine and verapamil displayed their binding into S2 sites, which are the thioredoxin-like fold present in each CASQ domain. Docking predictions combined with dissociation constants imply that presence of large aromatic cores and less flexible functional groups determines the strength of binding affinity to CASQ. In addition, the predicted binding pockets for both caffeine and epigallocatechin overlapped with the S1 and S2 sites, suggesting competitive inhibition by these natural compounds as a plausible explanation for their antagonistic effects on cardiotoxic side effects.
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Details
- Title
- Molecular Mechanisms of Pharmaceutical Drug Binding into Calsequestrin
- Creators
- Arun K Subra - Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA; E-MailsMark S Nissen - Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA; E-MailsKevin M Lewis - Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA; E-MailsAshwin K Muralidharan - Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA; E-MailsEmiliano J Sanchez - School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660, USA; E-MailHendrik Milting - Herz- und Diabeteszentrum NRW, Klinik der Ruhr Universitaet Bochum, Erich and Hanna Klessmann-Institut für Kardiovaskuläre Forschung und Entwicklung, 32545 Bad Oeynhausen, Germany; E-MailChulHee Kang - Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA; E-Mails
- Publication Details
- International journal of molecular sciences, Vol.13(11), pp.14326-14343
- Academic Unit
- Chemistry, Department of
- Publisher
- Molecular Diversity Preservation International (MDPI)
- Identifiers
- 99900546982301842
- Language
- English
- Resource Type
- Journal article