Journal article
Molecular and Anatomical Signatures of Sleep Deprivation in the Mouse Brain
Frontiers in neuroscience, Vol.4, pp.165-165
2010
Handle:
https://hdl.handle.net/2376/106289
PMCID: PMC2981377
PMID: 21088695
Abstract
Sleep deprivation (SD) leads to a suite of cognitive and behavioral impairments, and yet the molecular consequences of SD in the brain are poorly understood. Using a systematic immediate-early gene (IEG) mapping to detect neuronal activation, the consequences of SD were mapped primarily to forebrain regions. SD was found to both induce and suppress IEG expression (and thus neuronal activity) in subregions of neocortex, striatum, and other brain regions. Laser microdissection and cDNA microarrays were used to identify the molecular consequences of SD in seven brain regions.
In situ
hybridization (ISH) for 222 genes selected from the microarray data and other sources confirmed that robust molecular changes were largely restricted to the forebrain. Analysis of the ISH data for 222 genes (publicly accessible at
http://sleep.alleninstitute.org
) provided a molecular and anatomic signature of the effects of SD on the brain. The suprachiasmatic nucleus (SCN) and the neocortex exhibited differential regulation of the same genes, such that in the SCN genes exhibited time-of-day effects while in the neocortex, genes exhibited only SD and waking (W) effects. In the neocortex, SD activated gene expression in areal-, layer-, and cell type-specific manner. In the forebrain, SD preferentially activated excitatory neurons, as demonstrated by double-labeling, except for striatum which consists primarily of inhibitory neurons. These data provide a characterization of the anatomical and cell type-specific signatures of SD on neuronal activity and gene expression that may account for the associated cognitive and behavioral effects.
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Details
- Title
- Molecular and Anatomical Signatures of Sleep Deprivation in the Mouse Brain
- Creators
- Carol L Thompson - Allen Institute for Brain ScienceJonathan P Wisor - Biosciences Division, SRI InternationalChang-Kyu Lee - Allen Institute for Brain ScienceSayan D Pathak - Allen Institute for Brain ScienceDmitry Gerashchenko - Biosciences Division, SRI InternationalKimberly A Smith - Allen Institute for Brain ScienceShanna R Fischer - Allen Institute for Brain ScienceChihchau L Kuan - Allen Institute for Brain ScienceSusan M Sunkin - Allen Institute for Brain ScienceLydia L Ng - Allen Institute for Brain ScienceChristopher Lau - Allen Institute for Brain ScienceMichael Hawrylycz - Allen Institute for Brain ScienceAllan R Jones - Allen Institute for Brain ScienceThomas S Kilduff - Biosciences Division, SRI InternationalEdward S Lein - Allen Institute for Brain Science
- Publication Details
- Frontiers in neuroscience, Vol.4, pp.165-165
- Academic Unit
- Biomedical Sciences, Department of
- Publisher
- Frontiers Research Foundation
- Identifiers
- 99900546961601842
- Language
- English
- Resource Type
- Journal article