Journal article
Molecular and chemical dialogues in bacteria-protozoa interactions
Scientific reports, Vol.5(1), pp.12837-12837
08/06/2015
Handle:
https://hdl.handle.net/2376/107816
PMCID: PMC4542665
PMID: 26246193
Abstract
Protozoan predation of bacteria can significantly affect soil microbial community composition and ecosystem functioning. Bacteria possess diverse defense strategies to resist or evade protozoan predation. For soil-dwelling Pseudomonas species, several secondary metabolites were proposed to provide protection against different protozoan genera. By combining whole-genome transcriptome analyses with (live) imaging mass spectrometry (IMS), we observed multiple changes in the molecular and chemical dialogues between Pseudomonas fluorescens and the protist Naegleria americana. Lipopeptide (LP) biosynthesis was induced in Pseudomonas upon protozoan grazing and LP accumulation transitioned from homogeneous distributions across bacterial colonies to site-specific accumulation at the bacteria-protist interface. Also putrescine biosynthesis was upregulated in P. fluorescens upon predation. We demonstrated that putrescine induces protozoan trophozoite encystment and adversely affects cyst viability. This multifaceted study provides new insights in common and strain-specific responses in bacteria-protozoa interactions, including responses that contribute to bacterial survival in highly competitive soil and rhizosphere environments.
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Details
- Title
- Molecular and chemical dialogues in bacteria-protozoa interactions
- Creators
- Chunxu Song - 1] Laboratory of Phytopathology, Wageningen University, 6708 PB Wageningen, the Netherlands Microbial Ecology Department, Netherlands Institute of Ecology (NIOO-KNAW), 6708 PB Wageningen, the NetherlandsMark Mazzola - USDA-ARS, 1104 N. Western Ave., Wenatchee, Washington 98801Xu Cheng - Laboratory of Phytopathology, Wageningen University, 6708 PB Wageningen, the NetherlandsJanina Oetjen - Center for Industrial Mathematics, University of Bremen, 28359 Bremen, GermanyTheodore Alexandrov - 1] MALDI Imaging Lab, University of Bremen, 28359 Bremen, Germany Center for Industrial Mathematics, University of Bremen, 28359 Bremen, Germany SCiLS GmbH, 28359 Bremen, Germany Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego , San Diego, California 92093, United States Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, GermanyPieter Dorrestein - Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego , San Diego, California 92093, United StatesJeramie Watrous - Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego , San Diego, California 92093, United StatesMenno van der Voort - Laboratory of Phytopathology, Wageningen University, 6708 PB Wageningen, the NetherlandsJos M Raaijmakers - 1] Laboratory of Phytopathology, Wageningen University, 6708 PB Wageningen, the Netherlands Microbial Ecology Department, Netherlands Institute of Ecology (NIOO-KNAW), 6708 PB Wageningen, the Netherlands
- Publication Details
- Scientific reports, Vol.5(1), pp.12837-12837
- Academic Unit
- Plant Pathology, Department of
- Publisher
- England
- Identifiers
- 99900547556901842
- Language
- English
- Resource Type
- Journal article