Journal article
Molecular basis for surface antigen size polymorphisms and conservation of a neutralization-sensitive epitope in Anaplasma marginale
Proceedings of the National Academy of Sciences - PNAS, Vol.87(8), pp.3220-3224
04/1990
Handle:
https://hdl.handle.net/2376/114330
PMCID: PMC53867
PMID: 1691504
Abstract
Anaplasmosis is one of several tick-borne diseases severely constraining cattle production and usage in many parts of the world. Cattle can be protected from anaplasmosis by immunization with major surface protein 1, a surface protein of Anaplasma marginale carrying a neutralization-sensitive epitope. Marked size polymorphisms exist among different isolates of A. marginale in the AmF105 subunit of major surface protein 1, yet all isolates still contain the neutralization-sensitive epitope. To clarify the basis for these observations, the mspl alpha gene encoding AmF105 was cloned from four isolates and sequenced. The encoded polypeptides share a high degree of overall homology between isolates but contain a domain with various numbers of tandemly repeated sequences and three regions of clustered amino acid substitutions outside the repeat domain. The polypeptide size differences are completely explained by the variations in the numbers of tandem repeat units. We have mapped the neutralization-sensitive epitope to a sequence that is present within each repeat unit. These results identify a basis for size polymorphisms of the surface polypeptide antigen concomitant with B-cell epitope conservation in rickettsiae.
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Details
- Title
- Molecular basis for surface antigen size polymorphisms and conservation of a neutralization-sensitive epitope in Anaplasma marginale
- Creators
- D R Allred - Department of Infectious Diseases, University of Florida, Gainesville 32610T C McGuire - Department of Infectious Diseases, University of Florida, Gainesville 32610G H Palmer - Department of Infectious Diseases, University of Florida, Gainesville 32610S R Leib - Department of Infectious Diseases, University of Florida, Gainesville 32610T M Harkins - Department of Infectious Diseases, University of Florida, Gainesville 32610T F McElwain - Department of Infectious Diseases, University of Florida, Gainesville 32610A F Barbet - Department of Infectious Diseases, University of Florida, Gainesville 32610
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.87(8), pp.3220-3224
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Identifiers
- 99900547815401842
- Language
- English
- Resource Type
- Journal article