Journal article
Molecular cloning, characterization and in vitro expression of SERPIN B1 of bighorn sheep ( Ovis canadensis) and domestic sheep ( Ovis aries), and comparison with that of other species
Veterinary immunology and immunopathology, Vol.137(3), pp.327-331
2010
Handle:
https://hdl.handle.net/2376/106616
PMID: 20591503
Abstract
Mannheimia haemolytica infection results in enhanced PMN-mediated tissue damage in the lungs of bighorn sheep (BHS) compared to that of domestic sheep (DS). SERPIN B1 is an inhibitor of PMN-derived serine proteases. It prevents lung tissue injury by inhibiting the serine proteases released as a result of PMN lysis and degranulation. It is conceivable that PMNs of BHS exhibit decreased quantity and/or activity of SERPIN B1 which results in enhanced tissue injury and decreased bacterial clearance in pneumonic lungs of BHS. The objective of this study was to clone and express SERPIN B1 of BHS and DS, and develop antibodies to facilitate quantification of SERPIN B1. The 1,134
bp cDNA of SERPIN B1 of BHS and DS encodes a polypeptide of 377 amino acids. SERPIN B1 of BHS and DS exhibits 100% identity at the nucleotide and amino acid levels. The amino acid sequence of ovine (BHS/DS) SERPIN B1 displays 69%, 71%, 74%, 78% and 80% identity with that of rats, dogs, mice, humans and horses, respectively. Ovine SERPIN B1 expressed in
Escherichia coli was used to develop polyclonal antibodies in mice. Western blot analysis revealed the specificity of these antibodies for ovine rSERPIN B1.
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Details
- Title
- Molecular cloning, characterization and in vitro expression of SERPIN B1 of bighorn sheep ( Ovis canadensis) and domestic sheep ( Ovis aries), and comparison with that of other species
- Creators
- Renuka Subramaniam - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, USARohana P Dassanayake - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, USAJunzo Norimine - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, USAWendy C Brown - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, USADonald P Knowles - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, USASubramaniam Srikumaran - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, USA
- Publication Details
- Veterinary immunology and immunopathology, Vol.137(3), pp.327-331
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546972601842
- Language
- English
- Resource Type
- Journal article