Journal article
Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat
Theoretical and applied genetics, Vol.126(2), pp.523-533
02/2013
Handle:
https://hdl.handle.net/2376/110450
PMID: 23090143
Abstract
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most damaging diseases of wheat worldwide. It is essential to identify new genes for effective resistance against the disease. Durum wheat PI 480148, originally from Ethiopia, was resistant in all seedling tests with several predominant Pst races in the US under controlled greenhouse conditions and at multiple locations subject to natural infection for several years. To map the resistance gene(s) and to transfer it to common wheat, a cross was made between PI 480148 and susceptible common wheat genotype Avocet S (AvS). Resistant F3 plants with 42 chromosomes were selected cytologically and by testing with Pst race PST-100. A total of 157 F4 plants from a single F3 plant with 2n = 42 tested with PST-100 segregated in a 3 resistant: 1 susceptible ratio, indicating that a single dominant gene from PI 480148 conferred resistance. Using the F3:4 population and the resistance gene-analog polymorphism (RGAP) and simple sequence repeat (SSR) markers, the gene was mapped to the long arm of chromosome 2B. SSR marker Xwmc441 and RGAP marker XLRRrev/NLRRrev
350
flanked the resistance gene by 5.6 and 2.7 cM, respectively. The effective resistance of the gene to an Australian Pst isolate virulent to Yr5, which is also located on 2BL and confers resistance to all US Pst races, together with an allelism test of the two genes, indicated that the gene from PI 480148 is different from Yr5 and should be a new and useful gene for resistance to stripe rust. Resistant common wheat lines with plant types similar to AvS were selected for use in breeding programs.
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Details
- Title
- Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat
- Creators
- L Xu - Department of Plant Pathology Washington State University Pullman WA 99164-6430 USAM Wang - Department of Plant Pathology Washington State University Pullman WA 99164-6430 USAP Cheng - Department of Plant Pathology Washington State University Pullman WA 99164-6430 USAZ Kang - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture Northwest A&F University Yangling 712100 Shaanxi People’s Republic of ChinaS Hulbert - Department of Plant Pathology Washington State University Pullman WA 99164-6430 USAX Chen - Wheat Genetics, Quality, Physiology and Disease Research Unit US Department of Agriculture-Agricultural Research Service (USDA-ARS) Pullman WA 99164-6430 USA
- Publication Details
- Theoretical and applied genetics, Vol.126(2), pp.523-533
- Academic Unit
- Plant Pathology, Department of
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900547246001842
- Language
- English
- Resource Type
- Journal article