Journal article
Molecular requirement for sterols in herpes simplex virus entry and infectivity
Journal of virology, Vol.88(23), pp.13918-13922
12/2014
Handle:
https://hdl.handle.net/2376/103124
PMCID: PMC4248969
PMID: 25231306
Abstract
Herpes simplex virus 1 (HSV-1) required cholesterol or desmosterol for virion-induced membrane fusion. HSV successfully entered DHCR24(-/-) cells, which lack a desmosterol-to-cholesterol conversion enzyme, indicating that entry can occur independently of cholesterol. Depletion of desmosterol from these cells resulted in diminished HSV-1 entry, suggesting a general sterol requirement for HSV-1 entry and that desmosterol can operate in virus entry. Cholesterol functioned more effectively than desmosterol, suggesting that the hydrocarbon tail of cholesterol influences viral entry.
Metrics
12 Record Views
Details
- Title
- Molecular requirement for sterols in herpes simplex virus entry and infectivity
- Creators
- George A Wudiri - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, USASuzanne M Pritchard - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USAHong Li - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA Animal Disease Research Unit, USDA Agricultural Research Service, Washington State University, Pullman, Washington, USAJin Liu - School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington, USAHector C Aguilar - Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, USAStacey D Gilk - Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USAAnthony V Nicola - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA nicola@vetmed.wsu.edu
- Publication Details
- Journal of virology, Vol.88(23), pp.13918-13922
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Mechanical and Materials Engineering, School of
- Publisher
- United States
- Grant note
- T32 AI007025 / NIAID NIH HHS AI007025 / NIAID NIH HHS R01 AI109022 / NIAID NIH HHS R21 AI096103 / NIAID NIH HHS AI094329 / NIAID NIH HHS R21 AI094329 / NIAID NIH HHS AI096103 / NIAID NIH HHS
- Identifiers
- 99900546677301842
- Language
- English
- Resource Type
- Journal article