Journal article
Molecular structure and organization of the wheat genomic manganese superoxide dismutase gene
Genome, Vol.49(3), pp.209-218
03/2006
Handle:
https://hdl.handle.net/2376/113036
PMID: 16604103
Abstract
The genomic structure of a manganese superoxide dismutase (MnSOD) gene in wheat was elucidated by sequencing a clone from a BAC library of a stripe rust resistant wheat line. The clone was identified by hybridization with a wheat MnSOD cDNA. The gene consisted of 6 exons interrupted by 5 introns with a total length of 4770 nucleotides from the start codon to the termination codon. The wheat MnSOD gene was the longest among those sequenced from plant species. The transcription initiation site was preceded by a G+C-rich promoter without a TATA or CAAT box. The promoter contained many putative cis-acting regulatory elements, including an abscisic acid (ABA)-responsive element, a stress-responsive element, and a GC-repeat, as well as several other structural features in common with the promoter of the rice MnSOD gene. A Stowaway-like transposable element was found in intron 5 of the wheat MnSOD gene, but further investigation revealed the transposable element was not present in all copies of the MnSOD genes.
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Details
- Title
- Molecular structure and organization of the wheat genomic manganese superoxide dismutase gene
- Creators
- Kwang-Hyun Baek - Department of Crop and Soil Sciences, 210 Johnson Hall, Washington State University, Pullman, WA 99164-6420, USADaniel Z SkinnerPeng LingXianming Chen
- Publication Details
- Genome, Vol.49(3), pp.209-218
- Academic Unit
- Crop and Soil Sciences, Department of; Plant Pathology, Department of
- Publisher
- Canada
- Identifiers
- 99900547583001842
- Language
- English
- Resource Type
- Journal article