Journal article
Monoclonal Antibodies to the Equine CD2 T Lymphocyte Marker, to a Pan-Granulocyte/Monocyte Marker and to a Unique Pan-B Lymphocyte Marker
Immunobiology (1979), Vol.192(1), pp.48-64
1994
Handle:
https://hdl.handle.net/2376/114685
PMID: 7750989
Abstract
Murine monoclonal antibodies, HB88A, B29A and DH59B separately identify the CD2 T lymphocyte molecule, a unique pan-B lymphocyte surface marker and a pan-granulocyte/monocyte surface molecule, respectively, in the horse. Specificity was shown by two-color immunofluorescent flow cytometry and immunofluorescent microscopy. MAb HB88A reacted with a 52 kDa pan-T lymphocyte molecule present on 75 % ± 7 of peripheral blood lymphocytes (PBL) (n =15 horses). It also reacted with lymphocytes restricted to T lymphocyte dependent areas of lymph node and spleen. Specificity of mAb HB88A to CD2 was demonstrated by its reactivity to COS 7 cells which expressed a transfected 1.5 kb equine lymphocyte c-DNA clone having 77.5 % overall sequence homology with human CD2 c-DNA. MAb B29A reacted with a pan-B lymphocyte specific cell surface complex, 143, 72, 50, 40, 27 and 14.5 kDa, present on 19 % ± 7 of PBL (n = 15 horses). This complex has not been described in the horse or other species. MAb DH59B reacted with a 96 kDa pan-granulocyte/monocyte specific surface protein and identified macrophages and Kupffer cells in equine tissue sections. Together these mAbs can be used to identify and quantitate the major constituents of equine leukocytes.
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Details
- Title
- Monoclonal Antibodies to the Equine CD2 T Lymphocyte Marker, to a Pan-Granulocyte/Monocyte Marker and to a Unique Pan-B Lymphocyte Marker
- Creators
- Daniel B Tumas - Department of Veterinary Microbiology and PathologyAlbert L Brassfield - Department of Veterinary Microbiology and PathologyAngela S Travenor - Department of Immunology, AFRC Babraham Institute, Babraham, Cambridge, United KingdomMelissa T Hines - Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington, USAWilliam C Davis - Department of Veterinary Microbiology and PathologyTravis C McGuire - Department of Veterinary Microbiology and Pathology
- Publication Details
- Immunobiology (1979), Vol.192(1), pp.48-64
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier GmbH
- Identifiers
- 99900547656901842
- Language
- English
- Resource Type
- Journal article