Journal article
Monte Carlo simulation of single-cell irradiation by an electron microbeam
Radiation and environmental biophysics, Vol.39(3), pp.173-177
09/2000
Handle:
https://hdl.handle.net/2376/113831
PMID: 11095147
Abstract
A model is presented for irradiation of a cellular monolayer by an electron microbeam. Results are presented for two possible window designs, cells plated on the vacuum-isolation window and cells plated on Mylar above the vacuum-isolation window. Even for the thicker dual-membrane window that facilitates tissue culture and allows the target cell to be centered relative to the electron beam, the majority of the calculated beam spreading was contained in a volume typical of the mammalian HeLa cell line. None of the 104 electrons simulated at 25 keV were scattered into the spatial region occupied by neighbors of the target cell. Dose leakage was largest at 50 keV where the mean energy deposited in all neighbors was 21% of that deposited in the target cell. This ratio was reduced to 5% at 90 keV, the highest beam energy simulated. Lineal energy spectra of energy deposition events scored in the nucleus of the target cell became progressively more like the gamma-ray spectrum as the electron beam energy increased. Hence, our simulations provide strong support for the feasibility of a low-LET, single-cell irradiator.
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Details
- Title
- Monte Carlo simulation of single-cell irradiation by an electron microbeam
- Creators
- J. H Miller - Pacific Northwest National Laboratory, PO Box 999, Mail Stop K1-83, Richland, WA 99352, USA e-mail: john.h.miller@pnl.gov; Tel.: +1-509-3753907, Fax: +1-509-3756631 USM Sowa Resat - Pacific Northwest National Laboratory, PO Box 999, Mail Stop K1-83, Richland, WA 99352, USA e-mail: john.h.miller@pnl.gov; Tel.: +1-509-3753907, Fax: +1-509-3756631 USN. F Metting - Pacific Northwest National Laboratory, PO Box 999, Mail Stop K1-83, Richland, WA 99352, USA e-mail: john.h.miller@pnl.gov; Tel.: +1-509-3753907, Fax: +1-509-3756631 USK Wei - Washington State University Tri-Cities, Richland, WA 99352, USA USD. J Lynch - Washington State University Tri-Cities, Richland, WA 99352, USA USW. E Wilson - Washington State University Tri-Cities, Richland, WA 99352, USA US
- Publication Details
- Radiation and environmental biophysics, Vol.39(3), pp.173-177
- Academic Unit
- Engineering and Applied Sciences (TRIC), School of
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900548382401842
- Language
- English
- Resource Type
- Journal article