Movement and nucleocapsid proteins coded by two tospovirus species interact through multiple binding regions in mixed infections

Diwaker Tripathi; Gaurav Raikhy; Hanu R Pappu

doi:10.1016/j.virol.2015.01.009

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Movement and nucleocapsid proteins coded by two tospovirus species interact through multiple binding regions in mixed infections

Journal article

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Peer reviewed

Movement and nucleocapsid proteins coded by two tospovirus species interact through multiple binding regions in mixed infections

Diwaker Tripathi, Gaurav Raikhy and Hanu R Pappu

Virology (New York, N.Y.), Vol.478, pp.137-147

04/2015

DOI: https://doi.org/10.1016/j.virol.2015.01.009

Handle:

https://hdl.handle.net/2376/105192

PMID: 25666522

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Abstract

Two-Hybrid System Techniques

Plant Viral Movement Proteins - metabolism

Nucleocapsid Proteins - metabolism

Virus Replication

Centrifugation

Tobacco - virology

Protein Binding

beta-Galactosidase - analysis

Genes, Reporter

Protein Interaction Mapping

Tospovirus - physiology

Negative-stranded tospoviruses (family: Bunyaviridae) are among the most agronomically important viruses. Some of the tospoviruses are known to exist as mixed infections in the same host plant. Iris yellow spot virus (IYSV) and Tomato spotted wilt virus (TSWV) were used to study virus-virus interaction in dually infected host plants. Viral genes of both viruses were separately cloned into binary pSITE-BiFC vectors. BiFC results showed that the N and NSm proteins of IYSV interact with their counterparts coded by TSWV in dually infected Nicotiana benthamiana plants. BiFC results were further confirmed by pull down and yeast-2-hybrid (Y2H) assays. Interacting regions of the N and NSm proteins were also identified by Y2H system and β-galactosidase activity. Several regions of the N and NSm were found interacting with each other. The regions involved in these interactions are presumed to be critical for the functioning of the tospovirus N and NSm proteins. This is the first report of in vivo protein interactions of distinct tospoviruses in mixed infection.

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Title: Movement and nucleocapsid proteins coded by two tospovirus species interact through multiple binding regions in mixed infections
Creators: Diwaker Tripathi - Department of Plant Pathology, Washington State University, P.O. Box 646430, Pullman, WA 99164-6430, USA
Gaurav Raikhy - Department of Plant Pathology, Washington State University, P.O. Box 646430, Pullman, WA 99164-6430, USA
Hanu R Pappu - Department of Plant Pathology, Washington State University, P.O. Box 646430, Pullman, WA 99164-6430, USA. Electronic address: hrp@wsu.edu
Publication Details: Virology (New York, N.Y.), Vol.478, pp.137-147
Academic Unit: Plant Pathology, Department of
Publisher: United States
Identifiers: 99900546738701842
Language: English
Resource Type: Journal article