Journal article
Mu1-related transposable elements of maize preferentially insert into low copy number DNA
Genetics (Austin), Vol.140(1), pp.315-324
05/1995
Handle:
https://hdl.handle.net/2376/113377
PMCID: PMC1206558
PMID: 7635296
Abstract
The Mutator transposable element system of maize was originally identified through its induction of mutations at an exceptionally high frequency and at a wide variety of loci. The Mu1 subfamily of transposable elements within this system are responsible for the majority of Mutator-induced mutations. Mu 1-related elements were isolated from active Mutator plants and their flanking DNA was characterized. Sequence analyses revealed perfect nine base target duplications directly flanking the insert for 13 of the 14 elements studied. Hybridizational studies indicated that Mu1-like elements insert primarily into regions of the maize genome that are of low copy number. This preferential selection of low copy number DNA as targets for Mu element insertion was not directed by any specific secondary structure(s) that could be detected in this study, but the 9-bp target duplications exhibited a discernibly higher than random match with the consensus sequence 5'-G-T-T-G-G/C-A-G-G/A-G-3'.
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Details
- Title
- Mu1-related transposable elements of maize preferentially insert into low copy number DNA
- Creators
- A D Cresse - Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USAS H HulbertW E BrownJ R LucasJ L Bennetzen
- Publication Details
- Genetics (Austin), Vol.140(1), pp.315-324
- Academic Unit
- Plant Pathology, Department of
- Publisher
- United States
- Grant note
- AI-27713 / NIAID NIH HHS
- Identifiers
- 99900548294901842
- Language
- English
- Resource Type
- Journal article