Journal article
Multi-Site Modulation of Flux during Monolignol Formation in Loblolly Pine (Pinus taeda)
Biochemical and biophysical research communications, Vol.261(3), pp.652-657
08/11/1999
Handle:
https://hdl.handle.net/2376/107999
PMID: 10441481
Abstract
Loblolly pine (Pinus taeda L.) cell suspension cultures secrete monolignols when placed in 8% sucrose/20 mM KI solution, and these were used to identify phenylpropanoid pathway flux-modulating steps. When cells were provided with increasing amounts of either phenylalanine (Phe) or cinnamic acid, cellular concentrations of immediate downstream products (cinnamic and p-coumaric acids, respectively) increased, whereas caffeic and ferulic acid pool sizes were essentially unaffected. Increasing Phe concentrations resulted in increased amounts of p-coumaryl alcohol relative to coniferyl alcohol. However, exogenously supplied cinnamic, p-coumaric, caffeic, and ferulic acids resulted only in increases in their intercellular concentrations, but not that of downstream cinnamyl aldehydes and monolignols. Supplying p-coumaryl and coniferyl aldehydes up to 40,000–320,000-fold above the detection limits resulted in rapid, quantitative conversion into the monolignols. Only at nonphysiological concentrations was transient accumulation of intracellular aldehydes observed. These results indicate that cinnamic and p-coumaric acid hydroxylations assume important regulatory positions in phenylpropanoid metabolism, whereas cinnamyl aldehyde reduction does not serve as a control point.
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Details
- Title
- Multi-Site Modulation of Flux during Monolignol Formation in Loblolly Pine (Pinus taeda)
- Creators
- Aldwin M AnterolaHendrik van RensburgPieter S van HeerdenLaurence B DavinNorman G Lewis
- Publication Details
- Biochemical and biophysical research communications, Vol.261(3), pp.652-657
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547197001842
- Language
- English
- Resource Type
- Journal article