Journal article
Multi-primer target PCR for rapid identification of bovine DRB3 alleles
Animal genetics, Vol.32(4), pp.219-221
08/2001
Handle:
https://hdl.handle.net/2376/112940
PMID: 11531702
Abstract
Multi-primer target polymerase chain reaction (MPT-PCR) is a rapid method for the identification of specific BoLA-DRB3 alleles. In a single PCR reaction, the presence of two alleles associated with increased risk, DRB3.2*23 (DRB3*2701-2703, 2705-2707) and decreased risk, DRB3.2*16 (DRB3*1501, 1502), of mastitis in Canadian Holstein can be detected. Two outer primers amplify exon 2 of DRB3. Simultaneously, two inner, allele-specific primers amplify individual alleles. Initially, 40 cows previously typed by PCR-restriction fragment length polymorphism (PCR-RFLP) were genotyped using the multi-primer approach. An additional 30 cows were first genotyped by multi-primer target PCR, then by PCR-RFLP. All animals were correctly identified and there were no false positives. This technique can readily be modified to identify other BoLA alleles of interest.
Metrics
11 Record Views
Details
- Title
- Multi-primer target PCR for rapid identification of bovine DRB3 alleles
- Creators
- S A Ledwidge - Center for the Genetic Improvement of Livestock, Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1. sledwidg@uoguelph.caB A MallardJ P GibsonG B JansenZ H Jiang
- Publication Details
- Animal genetics, Vol.32(4), pp.219-221
- Academic Unit
- Animal Sciences, Department of
- Publisher
- England
- Identifiers
- 99900547872001842
- Language
- English
- Resource Type
- Journal article