Multigene engineering: dawn of an exciting new era in biotechnology

Henry Daniell; Amit Dhingra

doi:10.1016/S0958-1669(02)00297-5

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Multigene engineering: dawn of an exciting new era in biotechnology

Journal article

Open access

Peer reviewed

Multigene engineering: dawn of an exciting new era in biotechnology

Henry Daniell and Amit Dhingra

Current opinion in biotechnology, Vol.13(2), pp.136-141

04/2002

DOI: https://doi.org/10.1016/S0958-1669(02)00297-5

Handle:

https://hdl.handle.net/2376/115265

PMCID: PMC3481857

PMID: 11950565

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https://doi.org/10.1016/S0958-1669(02)00297-5View

Published (Version of record) Open

Abstract

Genetic Engineering - methods

Multigene Family - genetics

Transformation, Genetic

Plant Development

Plants, Genetically Modified - genetics

Plants, Genetically Modified - growth & development

Transgenes - genetics

Gene Silencing

Oryza - enzymology

beta Carotene - metabolism

Biotechnology - methods

Biotechnology - trends

Plants - metabolism

Plants, Genetically Modified - metabolism

Oryza - genetics

Plants - genetics

Biopharmaceutics - methods

Chloroplasts - genetics

Development of a rice variety enriched in provitamin A, the accumulation of polyhydroxybutyrate polyester in Arabidopsis nuclear transgenic plants (with enzymes targeted to chloroplasts in both), and the expression of bacterial operons via the chloroplast genome are recent landmark achievements in multigene engineering. Hyper-expression of transgenes has resulted in the formation of insecticidal protein crystals or inclusion bodies of pharmaceutical proteins in transgenic chloroplasts, achieving the highest level of transgene expression ever reported in transgenic plants. These achievements illustrate the potential of multigene engineering to realize benefits of the post-genomic revolution.

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Title: Multigene engineering: dawn of an exciting new era in biotechnology
Creators: Henry Daniell - Department of Molecular Biology and Microbiology, University of Central Florida, 12722 Research Parkway, Orlando, Florida 32826-3227, USA. daniell@mail.ucf.edu
Amit Dhingra
Publication Details: Current opinion in biotechnology, Vol.13(2), pp.136-141
Academic Unit: Horticulture, Department of
Publisher: England
Grant note: R01 GM063879 / NIGMS NIH HHS
Identifiers: 99900548597101842
Language: English
Resource Type: Journal article