Journal article
Multilocus Variable-Number Tandem-Repeat Analysis and Plasmid Profiling To Study the Occurrence of blaCMY-2 within a Pulsed-Field Gel Electrophoresis-Defined Clade of Salmonella enterica Serovar Typhimurium
Applied and environmental microbiology, Vol.76(1), pp.69-74
01/2010
Handle:
https://hdl.handle.net/2376/117477
PMCID: PMC2798641
PMID: 19880639
Abstract
Salmonella enterica
serovar Typhimurium circulating in food animal populations and carrying resistance to antimicrobial agents represents a human health risk. Recently, a new clade of
S
. Typhimurium, WA-TYP035/187, was reported in cattle and humans in the Pacific Northwest, United States of America. The objective of this study was to describe a possible mechanism of acquisition of expanded-spectrum cephalosporin resistance in this clade. Ceftazidime resistance increased steadily among WA-TYP035/187 isolates, from 0% (0/2) in 1999 to 77.8% (28/36) in 2006 (χ
2
for linear trend,
P
value of <0.001). Among 112 bovine-source and 18 human-source isolates, 49 (43.8%) and 12 (66.7%) were resistant to ceftazidime, respectively. Multiple-locus variable-number tandem-repeat analysis (MLVA) and plasmid profiling suggested that resistance was acquired by multiple independent genetic events within the WA-TYP035/187 clade. Given the lack of an obvious reservoir in species other than cattle and a parallel rise in ceftiofur resistance in the bovine-specific serovar
S
almonella enterica
serovar Dublin in the same time frame and region, selection pressure due to the use of the expanded-spectrum cephalosporin drug ceftiofur in cattle is a likely factor driving the increasing cephalosporin resistance of WA-TYP035/187.
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Details
- Title
- Multilocus Variable-Number Tandem-Repeat Analysis and Plasmid Profiling To Study the Occurrence of blaCMY-2 within a Pulsed-Field Gel Electrophoresis-Defined Clade of Salmonella enterica Serovar Typhimurium
- Creators
- B Adhikari - AAHP, Field Disease Investigation Unit, Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington 99164T. E Besser - AAHP, Field Disease Investigation Unit, Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington 99164J. M Gay - AAHP, Field Disease Investigation Unit, Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington 99164L. K Fox - AAHP, Field Disease Investigation Unit, Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington 99164D. D Hancock - AAHP, Field Disease Investigation Unit, Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington 99164M. A Davis - AAHP, Field Disease Investigation Unit, Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington 99164
- Publication Details
- Applied and environmental microbiology, Vol.76(1), pp.69-74
- Academic Unit
- Veterinary Clinical Sciences, Department of; Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900548466501842
- Language
- English
- Resource Type
- Journal article