Journal article
Multilocus variable-number tandem-repeat analysis for subtyping Salmonella enterica serovar Gallinarum
Avian pathology, Vol.40(6), pp.559-564
12/2011
Handle:
https://hdl.handle.net/2376/103288
PMID: 22107089
Abstract
Salmonella enterica serovar Gallinarum causes a severe systemic disease, fowl typhoid, primarily in chickens and turkeys, and it remains a disease of worldwide significance. Multilocus variable-number tandem-repeat analysis (MLVA) has proved to be very useful for subtyping other Salmonella serovars. We describe the development of a simple MLVA assay for S. enterica serovar Gallinarum that is comparable with pulsed-field gel electrophoresis (PFGE) in resolution. The genome sequence of S. enterica serovar Gallinarum strain 287/91 was analysed for potential variable-number tandem repeats (VNTRs) and then polymerase chain reaction assays were developed to assess the variability of the loci. Four VNTR markers were selected and used in a multiplex fragment analysis assay. The stability of the VNTR markers was assessed by conducting in vitro passage experiments with two strains (95 clones per strain) over a 30-day period. A MLVA of 68 strains of S. enterica serovar Gallinarum based on the four VNTR loci distinguished 26 allelic profiles. The MLVA assay showed a Simpson's diversity index of 0.918, whereas PFGE analysis produced 23 patterns and had a diversity index of 0.874. Most importantly, the MLVA further discriminated strains having the same PFGE pattern. The MLVA assay is a highly discriminatory genotyping method for S. enterica serovar Gallinarum. Therefore, MLVA can be a useful addition to routine PFGE analysis for molecular epidemiological investigation of fowl typhoid.
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Details
- Title
- Multilocus variable-number tandem-repeat analysis for subtyping Salmonella enterica serovar Gallinarum
- Creators
- Min S Kang - National Veterinary Research & Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries, Anyang, Gyeonggi, Republic of Korea.kangmskr@korea.krYong K KwonJae Y OhDouglas R CallByung K AnEun A SongJeong Y KimEun G ShinMi J KimJun H KwonGab S Chung
- Publication Details
- Avian pathology, Vol.40(6), pp.559-564
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- England
- Identifiers
- 99900546690001842
- Language
- English
- Resource Type
- Journal article