Journal article
Multiple Novel Functions of Henipavirus O-glycans: The First O-glycan Functions Identified in the Paramyxovirus Family
PLoS pathogens, Vol.12(2), pp.e1005445-e1005445
02/2016
Handle:
https://hdl.handle.net/2376/106394
PMCID: PMC4750917
PMID: 26867212
Abstract
O-linked glycosylation is a ubiquitous protein modification in organisms belonging to several kingdoms. Both microbial and host protein glycans are used by many pathogens for host invasion and immune evasion, yet little is known about the roles of O-glycans in viral pathogenesis. Reportedly, there is no single function attributed to O-glycans for the significant paramyxovirus family. The paramyxovirus family includes many important pathogens, such as measles, mumps, parainfluenza, metapneumo- and the deadly Henipaviruses Nipah (NiV) and Hendra (HeV) viruses. Paramyxoviral cell entry requires the coordinated actions of two viral membrane glycoproteins: the attachment (HN/H/G) and fusion (F) glycoproteins. O-glycan sites in HeV G were recently identified, facilitating use of the attachment protein of this deadly paramyxovirus as a model to study O-glycan functions. We mutated the identified HeV G O-glycosylation sites and found mutants with altered cell-cell fusion, G conformation, G/F association, viral entry in a pseudotyped viral system, and, quite unexpectedly, pseudotyped viral F protein incorporation and processing phenotypes. These are all important functions of viral glycoproteins. These phenotypes were broadly conserved for equivalent NiV mutants. Thus our results identify multiple novel and pathologically important functions of paramyxoviral O-glycans, paving the way to study O-glycan functions in other paramyxoviruses and enveloped viruses.
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Details
- Title
- Multiple Novel Functions of Henipavirus O-glycans: The First O-glycan Functions Identified in the Paramyxovirus Family
- Creators
- Jacquelyn A Stone - Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, United States of AmericaAnthony V Nicola - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of AmericaLinda G Baum - Department of Pathology and Laboratory Medicine, University of California, Los Angeles, California, United States of AmericaHector C Aguilar - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of America
- Publication Details
- PLoS pathogens, Vol.12(2), pp.e1005445-e1005445
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- United States
- Grant note
- R01 AI109022 / NIAID NIH HHS T32 GM008336 / NIGMS NIH HHS R01 AI119159 / NIAID NIH HHS AI109022 / NIAID NIH HHS AI119159 / NIAID NIH HHS T32GM008336 / NIGMS NIH HHS
- Identifiers
- 99900546522701842
- Language
- English
- Resource Type
- Journal article