Multiple double-stranded RNA segments are associated with virus particles infecting Trichomonas vaginalis

A Khoshnan; J F Alderete

doi:10.1128/JVI.67.12.6950-6955.1993

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Multiple double-stranded RNA segments are associated with virus particles infecting Trichomonas vaginalis

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Multiple double-stranded RNA segments are associated with virus particles infecting Trichomonas vaginalis

A Khoshnan and J F Alderete

Journal of virology, Vol.67(12), pp.6950-6955

12/1993

DOI: https://doi.org/10.1128/JVI.67.12.6950-6955.1993

Handle:

https://hdl.handle.net/2376/110858

PMCID: PMC238153

PMID: 8230417

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https://doi.org/10.1128/JVI.67.12.6950-6955.1993View

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Abstract

RNA, Double-Stranded - isolation & purification

Blotting, Northern

Nucleic Acid Hybridization

RNA Viruses - isolation & purification

Animals

RNA Viruses - ultrastructure

Cloning, Molecular

DNA, Complementary - genetics

RNA Viruses - genetics

Trichomonas vaginalis - microbiology

Previous studies demonstrated that some isolates of the sexually transmitted protozoan Trichomonas vaginalis are infected with a nonsegmented, double-stranded RNA (dsRNA) virus. A reexamination of the total dsRNA extracted from several virus-harboring isolates indicated the presence of at least three dsRNAs with sizes ranging from 4.8 to 4.3 kbp. The double-stranded nature of each of the three segments was determined by hybridization experiments using riboprobes of opposite polarities obtained from cDNA generated to each of the segments. All three segments were present in agar clones originating from single organisms of T. vaginalis isolates, suggesting that the three segments were not the result of a mixed population of trichomonads harboring different sizes of dsRNA. The three segments were associated with CsCl-purified virus particles, as evidenced by electron microscopy, and RNAse treatment of the preparation containing virus particles did not destroy the dsRNAs. Finally, the individual dsRNA segments were purified for use as probes to determine whether the three dsRNAs shared any sequence homology. Each end-labeled dsRNA segment did not cross-hybridize to any of the other two segments, a finding consistent with the hybridization of labeled cDNAs to only the segments from which they were derived. These results show that the coding capacity of the dsRNA virus may be at least three times greater than that estimated earlier and illustrates further the complexity of this virus-parasite interrelationship.

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Title: Multiple double-stranded RNA segments are associated with virus particles infecting Trichomonas vaginalis
Creators: A Khoshnan - Department of Microbiology, University of Texas Health Science Center, San Antonio 78284-7758
J F Alderete
Publication Details: Journal of virology, Vol.67(12), pp.6950-6955
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: AI07271 / NIAID NIH HHS AI31498 / NIAID NIH HHS
Identifiers: 99900547237601842
Language: English
Resource Type: Journal article