Journal article
Multiple loci contribute to genome-wide recombination levels in male mice
Mammalian genome, Vol.21(11-12), pp.550-555
12/2010
Handle:
https://hdl.handle.net/2376/101733
PMCID: PMC3002158
PMID: 21113599
Abstract
Recent linkage-based studies in humans suggest the presence of loci that affect either genome-wide recombination rates, utilization of recombination hotspots, or both. We have been interested in utilizing cytological methodology to directly assess recombination in mammalian meiocytes and to identify recombination-associated loci. In the present report we summarize studies in which we combined a cytological assay of recombination in mouse pachytene spermatocytes with QTL analyses to identify loci that contribute to genome-wide levels of recombination in male meiosis. Specifically, we analyzed MLH1 foci, a marker of crossovers, in 194 F2 male mice derived from a subspecific cross between CAST/EiJ and C57BL/6J parental strains. We then used these data to uncover loci associated with individual variation in mean MLH1 values. We identified seven recombination-associated loci across the genome (on chromosomes 2, 3, 4, 14, 15, 17, and X), indicating that there are multiple recombination “setting” loci in mammalian male meiosis.
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Details
- Title
- Multiple loci contribute to genome-wide recombination levels in male mice
- Creators
- Brenda Murdoch - School of Molecular Biosciences, Washington State University, Pullman, WA 99164 USANichole Owen - School of Molecular Biosciences, Washington State University, Pullman, WA 99164 USASofia Shirley - School of Molecular Biosciences, Washington State University, Pullman, WA 99164 USASara Crumb - School of Molecular Biosciences, Washington State University, Pullman, WA 99164 USAKarl W Broman - Department of Biostatistics and Medical Informatics, University of Wisconsin-Madison, Madison, WI 53706 USATerry Hassold - School of Molecular Biosciences, Washington State University, Pullman, WA 99164 USA
- Publication Details
- Mammalian genome, Vol.21(11-12), pp.550-555
- Academic Unit
- Center for Reproductive Biology; Molecular Biosciences, School of
- Publisher
- Springer-Verlag; New York
- Identifiers
- 99900546797501842
- Language
- English
- Resource Type
- Journal article