Journal article
Multiple-targeted graphene-based nanocarrier for intracellular imaging of mRNAs
Analytica chimica acta, Vol.983(2017), pp.1-8
08/29/2017
Handle:
https://hdl.handle.net/2376/108523
PMID: 28811015
Abstract
Simultaneous detection and imaging of multiple intracellular messenger RNA (mRNAs) hold great significant for early cancer diagnostics and preventive medicine development. Herein, we propose a multiple-targeted graphene oxide (GO) nanocarrier that can simultaneously detect and image different type mRNAs in living cells. First of all, in vitro detection of multiple targets have been realized successfully based on the multiple-targeted GO nanocarrier with linear relationship ranging from 3 nM to 200 nM, as well as sensitive detection limit of 1.84 nM for manganese superoxide dismutase (Mn-SOD) mRNA and 2.45 nM for β-actin mRNA. Additionally, this nanosensing platform composed of fluorescent labelled single strand DNA probes and GO nanocarrier can identify Mn-SOD mRNA and endogenous mRNA of β-actin in living cancer cells, showing rapid response, high specificity, nuclease stability, and good biocompatibility during the cell imaging. Thirdly, changes of the expression levels of mRNA in living cells before or after the drug treatment can be monitored successfully. By using multiple ssDNA as probes and GO nanocarrier as the cellular delivery cargo, the proposed simultaneous multiple-targeted sensing platform will be of great potential as a powerful tool for intracellular trafficking process from basic research to clinical diagnosis.
Schematic illustration of simultaneously multiple mRNAs monitoring inside single living breast cancer cell based on GO nanocarrier. In particular, the fluorescent signals could be monitored when Mn-SOD probe (red) and β-actin probe (green) hybridizes with their mRNA targets inside the living cells. Random probe (orange) was regarded as control probe for the sensing strategy. [Display omitted]
•A multiple-targeted GO nanocarrier was used for mRNAs imaging and expression changes after drug treatment can be monitored successfully.•Sensitive detection limit of 1.84 nM for manganese superoxide dismutase (Mn-SOD) mRNA and 2.45 nM for β-actin mRNA was accomplished.•Changes of the expression levels of mRNA in living cells before or after the drug treatment can be monitored successfully.
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Details
- Title
- Multiple-targeted graphene-based nanocarrier for intracellular imaging of mRNAs
- Creators
- Ying Wang - Shanghai Key Lab of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, College of Environmental Science and Technology, Tongji University, Shanghai, 200092, ChinaZhaohui Li - College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, PR ChinaMisha Liu - Shanghai Key Lab of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, College of Environmental Science and Technology, Tongji University, Shanghai, 200092, ChinaJinjin Xu - Shanghai Key Lab of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, College of Environmental Science and Technology, Tongji University, Shanghai, 200092, ChinaDehong Hu - Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USAYuehe Lin - Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USAJinghong Li - Department of Chemistry, Tsinghua University, Beijing, 100084, PR China
- Publication Details
- Analytica chimica acta, Vol.983(2017), pp.1-8
- Academic Unit
- Chemical Engineering and Bioengineering, School of; Mechanical and Materials Engineering, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546918301842
- Language
- English
- Resource Type
- Journal article