Journal article
Multistage isoelectric focusing in a polymeric microfluidic chip
Analytical chemistry (Washington), Vol.77(24), pp.7878-7886
12/15/2005
Handle:
https://hdl.handle.net/2376/113663
PMID: 16351133
Abstract
This paper reports a protocol that improves the resolving power of isoelectric focusing (IEF) in a polymeric microfluidic chip. This method couples several stages of IEF in series by first focusing proteins in a straight channel using broad-range ampholytes and then refocusing segments of the first channel into secondary channels that branch from the first one at T-junctions. Experiments demonstrate that several fluorescent proteins that had focused within a segment of the straight channel in the first stage were refocused at significantly higher resolution due to the shallower pH gradient and higher electrical field gradient. Two variants of green fluorescent protein from the second-stage IEF fractionation were further separated in a third stage. Three stages of IEF were completed in less than 25 min at electric field strengths ranging from 50 to 214 V/cm.
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Details
- Title
- Multistage isoelectric focusing in a polymeric microfluidic chip
- Creators
- Huanchun Cui - Department of Chemical Engineering and School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, USAKeisuke HoriuchiPrashanta DuttaCornelius F Ivory
- Publication Details
- Analytical chemistry (Washington), Vol.77(24), pp.7878-7886
- Academic Unit
- Chemical Engineering and Bioengineering, School of; Mechanical and Materials Engineering, School of
- Publisher
- United States
- Identifiers
- 99900548215701842
- Language
- English
- Resource Type
- Journal article