Murine germ cells do not require functional androgen receptors to complete spermatogenesis following spermatogonial stem cell transplantation

D S Johnston; L D Russell; P J Friel; M D Griswold

doi:10.1210/en.142.6.2405

Back

Murine germ cells do not require functional androgen receptors to complete spermatogenesis following spermatogonial stem cell transplantation

Journal article

Open access

Peer reviewed

Murine germ cells do not require functional androgen receptors to complete spermatogenesis following spermatogonial stem cell transplantation

D S Johnston, L D Russell, P J Friel and M D Griswold

Endocrinology (Philadelphia), Vol.142(6), pp.2405-2408

06/2001

DOI: https://doi.org/10.1210/en.142.6.2405

Handle:

https://hdl.handle.net/2376/105028

PMID: 11356688

Files and links (1)

url

https://doi.org/10.1210/en.142.6.2405View

Published (Version of record) Open

Abstract

Testis - cytology

Spermatogenesis

Mice, Inbred C57BL

Oligospermia - genetics

Male

Stem Cell Transplantation

Animals

Receptors, Androgen - genetics

Gene Deletion

Polymerase Chain Reaction

Female

Spermatozoa - physiology

Cell Differentiation

Mice

Seminiferous Tubules - cytology

Spermatogonia - transplantation

Mutation

Spermatozoa - ultrastructure

Receptors, Androgen - physiology

The spermatogonial stem cell transplantation technique was employed to determine if murine germ cells require functional androgen receptors to complete qualitatively normal spermatogenesis. Testicular cells from testicular feminized mice were injected into the seminiferous tubules of azoospermic mice expressing functional androgen receptors. Recipient testes were analyzed between 110 and 200 days following transplantation. Multiple colonies of complete and qualitatively normal donor-derived spermatogenesis were seen within the seminiferous tubules of each recipient testis, demonstrating that murine germ cells do not require functional androgen receptors to complete spermatogenesis.

Metrics

13 Record Views

See more details

Details

Title: Murine germ cells do not require functional androgen receptors to complete spermatogenesis following spermatogonial stem cell transplantation
Creators: D S Johnston - School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman 99164, USA
L D Russell
P J Friel
M D Griswold
Publication Details: Endocrinology (Philadelphia), Vol.142(6), pp.2405-2408
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: HD08437 / NICHD NIH HHS HD35494 / NICHD NIH HHS
Identifiers: 99900546610701842
Language: English
Resource Type: Journal article