Journal article
Muscle growth in teleost fish is regulated by factors utilizing the activin II B receptor
Journal of experimental biology, Vol.216(19), pp.3742-3750
10/01/2013
PMID: 23788712
Abstract
The activin type IIB receptor (Acvr2b) is the cell surface receptor for multiple transforming growth factor beta (TGF-beta) superfamily ligands, several of which regulate muscle growth in mammals. To investigate the role of the Acvr2b signaling pathway in the growth and development of skeletal muscle in teleost fish, transgenic rainbow trout (RBT; Oncorhynchus mykiss) expressing a truncated form of the acvr2b-2a (acvr2b(Delta)) in muscle tissue were produced. High levels of acvr2b(Delta) expression were detected in the majority of P1 transgenic fish. Transgenic P1 trout developed enhanced, localized musculature in both the epaxial and hypaxial regions (dubbed 'six pack'). The F1 transgenic offspring did not exhibit localized muscle growth, but rather developed a uniform body morphology with greater girth, condition factor and increased muscle fiber hypertrophy. There was a high degree of variation in the mass of both P1 and F1 transgenic fish, with several fish of each generation exhibiting enhanced growth compared with other transgenic and control siblings. The 'six pack' phenotype observed in P1 transgenic RBT overexpressing acvr2b(Delta) and the presence of F1 individuals with altered muscle morphology provides compelling evidence for the importance of TGF-beta signaling molecules in regulating muscle growth in teleost fish.
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Details
- Title
- Muscle growth in teleost fish is regulated by factors utilizing the activin II B receptor
- Creators
- Michael P. Phelps - University of Rhode IslandIan M. Jaffe - University of Rhode IslandTerence M. Bradley - University of Rhode Island
- Publication Details
- Journal of experimental biology, Vol.216(19), pp.3742-3750
- Academic Unit
- Department of Animal Sciences
- Publisher
- COMPANY OF BIOLOGISTS LTD
- Number of pages
- 9
- Grant note
- National Institute of Food and Agriculture; United States Department of Agriculture (USDA); National Institute of Food & Agriculture H-899 / Rhode Island Agricultural Experiment Station 2009-35206-05288 / National Research Initiative Competitive from the United States Department of Agriculture 582477; 2009-35206-05288 / NIFA; United States Department of Agriculture (USDA); National Institute of Food & Agriculture
- Identifiers
- 99901398983001842
- Language
- English
- Resource Type
- Journal article