Journal article
Mutagenesis and evaluation of cellulase properties and cellulose hydrolysis of Talaromyces piceus
World journal of microbiology & biotechnology, Vol.31(11), pp.1811-1819
11/2015
Handle:
https://hdl.handle.net/2376/117707
PMID: 26330062
Abstract
A fungal species with a high yield of β-glucosidase was isolated and identified as Talaromyces piceus 9-3 (anamorph: Penicillium piceum) by morphological and molecular characterization. Through dimethyl sulphate mutagenesis, the cellulase over-producing strain T. piceus H16 was obtained. The FPase activity and β-glucosidase activity of T. piceus H16 were 5.83 and 53.12 IU ml(-1) respectively--a 5.34- and 4.43-times improvement from the parent strain T. piceus 9-3. The optimum pH and temperature for enzyme activity were pH 5.0 and 50 °C for FPase activity and pH 5.0 and 55 °C for β-glucosidase activity, respectively. The cellulase were quite stable at 37 °C, only losing <10% of their initial activity after 24 h of incubation. Hydrolysis analysis results showed that a highly efficient synergistic effect was achieved by combining cellulase from T. piceus H16 with that from Trichoderma reesei RUT C30 on hydrolyzing different substrates due to the high β-glucosidase activity of T. piceus H16. These data suggest that T. piceus H16 can be used as a potential cellulase producer with good prospects.
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Details
- Title
- Mutagenesis and evaluation of cellulase properties and cellulose hydrolysis of Talaromyces piceus
- Creators
- Ronglin He - Tianjin Key Lab of Industrial Microbiology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of ChinaPengli Cai - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of ChinaGaihong Wu - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of ChinaCan Zhang - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of ChinaDongyuan Zhang - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China. zhang_dy@tib.cas.cnShulin Chen - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China
- Publication Details
- World journal of microbiology & biotechnology, Vol.31(11), pp.1811-1819
- Identifiers
- 99900583062301842
- Language
- English
- Resource Type
- Journal article