Mutant Analysis in Arabidopsis Provides Insight into the Molecular Mode of Action of the Auxinic Herbicide Dicamba

Cynthia Gleason; Rhonda C Foley; Karam B Singh

doi:10.1371/journal.pone.0017245

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Mutant Analysis in Arabidopsis Provides Insight into the Molecular Mode of Action of the Auxinic Herbicide Dicamba

Journal article

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Mutant Analysis in Arabidopsis Provides Insight into the Molecular Mode of Action of the Auxinic Herbicide Dicamba

Cynthia Gleason, Rhonda C Foley and Karam B Singh

PloS one, Vol.6(3), pp.e17245-e17245

2011

DOI: https://doi.org/10.1371/journal.pone.0017245

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https://hdl.handle.net/2376/106439

PMCID: PMC3050828

PMID: 21408147

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Abstract

Agriculture

Biology

Herbicides that mimic the natural auxin indole-3-acetic acid are widely used in weed control. One common auxin-like herbicide is dicamba, but despite its wide use, plant gene responses to dicamba have never been extensively studied. To further understand dicamba's mode of action, we utilized Arabidopsis auxin-insensitive mutants and compared their sensitivity to dicamba and the widely-studied auxinic herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The mutant axr4-2 , which has disrupted auxin transport into cells, was resistant to 2,4-D but susceptible to dicamba. By comparing dicamba resistance in auxin signalling F-box receptor mutants ( tir1-1 , afb1 , afb2 , afb3 , and afb5 ), only tir1-1 and afb5 were resistant to dicamba, and this resistance was additive in the double tir1-1/afb5 mutant. Interestingly, tir1-1 but not afb5 was resistant to 2,4-D. Whole genome analysis of dicamba-induced gene expression showed that 10 hours after application, dicamba stimulated many stress-responsive and signalling genes, including those involved in biosynthesis or signalling of auxin, ethylene, and abscisic acid (ABA), with TIR1 and AFB5 required for the dicamba-responsiveness of some genes. Research into dicamba-regulated gene expression and the selectivity of auxin receptors has provided molecular insight into dicamba-regulated signalling and could help in the development of novel herbicide resistance in crop plants.

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Title: Mutant Analysis in Arabidopsis Provides Insight into the Molecular Mode of Action of the Auxinic Herbicide Dicamba
Creators: Cynthia Gleason
Rhonda C Foley
Karam B Singh
Publication Details: PloS one, Vol.6(3), pp.e17245-e17245
Academic Unit: Plant Pathology, Department of
Publisher: Public Library of Science; San Francisco, USA
Identifiers: 99900546813601842
Language: English
Resource Type: Journal article