Journal article
Mutation of essential Hsp90 co-chaperones SGT1 or CNS1 renders yeast hypersensitive to overexpression of other co-chaperones
Current genetics, Vol.60(4), pp.265-276
11/2014
Handle:
https://hdl.handle.net/2376/104007
PMID: 24923785
Abstract
The essential molecular chaperone Hsp90 functions with over ten co-chaperones in Saccharomyces cerevisiae, but the in vivo roles of many of these co-chaperones are poorly understood. Two of these co-chaperones, Cdc37 and Sgt1, target specific types of clients to Hsp90 for folding. Other co-chaperones have general roles in supporting Hsp90 function, but the degree of overlapping or competing functions is unclear. None of the chaperones, when overexpressed, were able to rescue the lethality of an SGT1 disruption strain. However, overexpression of SBA1, PPT1, AHA1 or HCH1 caused varying levels of growth defects in an sgt1-K360E strain. Negative effects of CPR6 overexpression were similarly observed in cells expressing the temperature-sensitive mutation cns1-G90D. In all cases, alterations within co-chaperones designed to disrupt Hsp90 interaction relieved the negative growth defects. Sgt1-K360E and Cns1-G90D were previously shown to exhibit reduced Hsp90 interaction. Our results indicate that overexpression of other co-chaperones further disrupts the essential functions of Cns1 and Sgt1. However, the specificity of the negative effects indicates that only a subset of co-chaperones competes with Sgt1 or Cns1 for binding to Hsp90. This provides new evidence that co-chaperones selectively compete for binding to subpopulations of cellular Hsp90 and suggest that changes in the relative levels of co-chaperones may have dramatic effects on Hsp90 function.
Metrics
6 Record Views
Details
- Title
- Mutation of essential Hsp90 co-chaperones SGT1 or CNS1 renders yeast hypersensitive to overexpression of other co-chaperones
- Creators
- Jill Johnson - Department of Biological Sciences University of Idaho P.O. Box 443051 Moscow ID 83844-3051 USAAbbey Zuehlke - Urologic Oncology Branch, Center for Cancer Research National Cancer Institute 9000 Rockville Pike Bethesda MD 20892 USAVictoria Tenge - Department of Biological Sciences University of Idaho P.O. Box 443051 Moscow ID 83844-3051 USAJordan Langworthy - Department of Biological Sciences University of Idaho P.O. Box 443051 Moscow ID 83844-3051 USA
- Publication Details
- Current genetics, Vol.60(4), pp.265-276
- Academic Unit
- UNKNOWN
- Publisher
- Springer Berlin Heidelberg; Berlin/Heidelberg
- Identifiers
- 99900546953701842
- Language
- English
- Resource Type
- Journal article