Journal article
Mutational analysis of two highly conserved motifs in the silencing suppressor encoded by tomato spotted wilt virus (genus Tospovirus, family Bunyaviridae)
Archives of virology, Vol.159(6), pp.1499-1504
06/2014
Handle:
https://hdl.handle.net/2376/107278
PMID: 24363189
Abstract
Tospoviruses cause serious economic losses to a wide range of field and horticultural crops on a global scale. The NSs gene encoded by tospoviruses acts as a suppressor of host plant defense. We identified amino acid motifs that are conserved in all of the NSs proteins of tospoviruses for which the sequence is known. Using tomato spotted wilt virus (TSWV) as a model, the role of these motifs in suppressor activity of NSs was investigated. Using site-directed point mutations in two conserved motifs, glycine, lysine and valine/threonine (GKV/T) at positions 181-183 and tyrosine and leucine (YL) at positions 412-413, and an assay to measure the reversal of gene silencing in Nicotiana benthamiana line 16c, we show that substitutions (K182 to A, and L413 to A) in these motifs abolished suppressor activity of the NSs protein, indicating that these two motifs are essential for the RNAi suppressor function of tospoviruses.
Metrics
8 Record Views
Details
- Title
- Mutational analysis of two highly conserved motifs in the silencing suppressor encoded by tomato spotted wilt virus (genus Tospovirus, family Bunyaviridae)
- Creators
- Ying Zhai - Department of Plant Pathology Washington State University PO Box 646430 Pullman WA 99164 USASudeep Bag - Department of Plant Pathology Washington State University PO Box 646430 Pullman WA 99164 USANeena Mitter - QAAFI University of Queensland St Lucia QLD AustraliaMassimo Turina - Istituto di Virologia Vegetale, CNR Strada delle Cacce 75 10135 Turin ItalyHanu Pappu - Department of Plant Pathology Washington State University PO Box 646430 Pullman WA 99164 USA
- Publication Details
- Archives of virology, Vol.159(6), pp.1499-1504
- Academic Unit
- Plant Pathology, Department of
- Publisher
- Springer Vienna; Vienna
- Identifiers
- 99900546805701842
- Language
- English
- Resource Type
- Journal article