Journal article
Mycoplasma ovipneumoniae can predispose bighorn sheep to fatal Mannheimia haemolytica pneumonia
Veterinary microbiology, Vol.145(3), pp.354-359
2010
Handle:
https://hdl.handle.net/2376/115246
PMID: 20466492
Abstract
Mycoplasma ovipneumoniae has been isolated from the lungs of pneumonic bighorn sheep (BHS). However experimental reproduction of fatal pneumonia in BHS with
M. ovipneumoniae was not successful. Therefore the specific role, if any, of
M. ovipneumoniae in BHS pneumonia is unclear. The objective of this study was to determine whether
M. ovipneumoniae alone causes fatal pneumonia in BHS, or predisposes them to infection by
Mannheimia haemolytica. We chose
M. haemolytica for this study because of its isolation from pneumonic BHS, and its consistent ability to cause fatal pneumonia under experimental conditions. Since
in vitro culture could attenuate virulence of
M. ovipneumoniae, we used ceftiofur-treated lung homogenates from pneumonic BHS lambs or nasopharyngeal washings from
M. ovipneumoniae-positive domestic sheep (DS) as the source of
M. ovipneumoniae. Two adult BHS were inoculated intranasally with lung homogenates while two others received nasopharyngeal washings from DS. All BHS developed clinical signs of respiratory infection, but only one BHS died. The dead BHS had carried leukotoxin-positive
M. haemolytica in the nasopharynx before the onset of this study. It is likely that
M. ovipneumoniae colonization predisposed this BHS to fatal infection with the
M. haemolytica already present in this animal. The remaining three BHS developed pneumonia and died 1–5 days following intranasal inoculation with
M. haemolytica. On necropsy, lungs of all four BHS showed lesions characteristic of bronchopneumonia.
M. haemolytica and
M. ovipneumoniae were isolated from the lungs. These results suggest that
M. ovipneumoniae alone may not cause fatal pneumonia in BHS, but can predispose them to fatal pneumonia due to
M. haemolytica infection.
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Details
- Title
- Mycoplasma ovipneumoniae can predispose bighorn sheep to fatal Mannheimia haemolytica pneumonia
- Creators
- Rohana P Dassanayake - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-6610, USASudarvili Shanthalingam - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-6610, USACaroline N Herndon - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-6610, USARenuka Subramaniam - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-6610, USAPaulraj K Lawrence - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-6610, USAJegarubee Bavananthasivam - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-6610, USAE. Frances Cassirer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-6610, USAGary J Haldorson - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-6610, USAWilliam J Foreyt - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-6610, USAFred R Rurangirwa - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-6610, USADonald P Knowles - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-6610, USAThomas E Besser - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-6610, USASubramaniam Srikumaran - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-6610, USA
- Publication Details
- Veterinary microbiology, Vol.145(3), pp.354-359
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548061201842
- Language
- English
- Resource Type
- Journal article